[Vor.8 
80 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
Since the dry weight determinations of Series 1-11 inclusive 
were equal within the limits of experimental error and the total 
amounts of culture solution remaining in the flasks after the 
TABLE II 
SHOWING THE ACTIVITY OF INTRA- AND EXTRACELLULAR AMYLASE 
PRODUCED BY FUSARIUM SP. WHEN GROWN IN CZAPEK'S SOLUTION 
HAVING INITIAL H-ION CONCENTRATIONS OF Pu 3.0 AND 4.5 
í Initial H-ion concentration of Czapek’s solution 
Px 3.0 Pu 4.5 
Ra Ce. N/50 KMnO, Pa of Ce. N/50 KMnO, 
buffer buffer 
sol.* sol.* 
Total | Contro] | Amylase Total | Contro] | Amylase 
activity activity 
3.0 2.9 0.4 1.8 3.0 4.3 1.3 3.0 
E 5.3 6.7 0.5 6.2 5.2 9.8 1.3 8.5 
z 3 eo | T1] 04]| €7]| 60] Oe) 14-41-08 
S| 7.0 6.0 0.4 5.6 7.0 11.0 1.3 9.7 
$ a| 8.0 4.0 0.6 3.4 8.0 8.5 1.5 7.0 
A 8.6 1.3 0.5 0.8 8.6 4.1 1.5 2.6 
11.0 0.7 0.5 0.2 | 11.0 1.5 1.6 0.0 . 
7.20 | 6.5 0.5 6.0 7.20 | 10.5 1.5 9.0 
3.0 9.2 2.8 6.4 3.2 6.6 2.6 4.0 
5.6 | 11.1 2.8 8.3 5.2 | 10.1 2.9 7.2 
E 6.0 | 13.2 3.0 | 10.2 6.0 | 10.8 2.9 7.9 
38| 7.0 | 11.8 2.8 9.0 6.9 | 10.1 2.9 7 
$u* 7.7 9.2 2.8 6.4 7.5 6.4 2.9 3.5 
EG! 8.2 6.1 2.9 3.2 8.7 2.8 2.6 0.2 
t 11.0 3.0 2.8 0.2 | 1L0 2.6 2.6 0.0 
6.0: | 13.3 2.8 | 10.5 6.1¢ | 9.9 2.5 7.4 
* H-ion concentration of the buffer solution in which enzyme activity was meas- 
ured. 
1 Doubly distilled water was substituted for the buffer solution. 
growth of the fungus were the same, the tables and curves can 
be compared directly. The amounts of enzyme produced in 
the culture solution according to the tables will thus represent 
