1921) i 
KARRER—H-ION CONCENTRATION AND AMYLASE ACTION 83 
W2 of the amylase excreted into the culture medium by one dry 
weight of the fungus. The amounts obtained with intracellular 
amylase will represent an average of about % of the total found 
in the mycelium of one culture. 
TABLE V 
SHOWING THE ACTIVITY OF INTRA- AND EXTRACELLULAR AMYLASE 
PRODUCED BY PENICILLIUM ITALICUM WHEN GROWN IN CZAPEK'S 
SOLUTION WITH H-ION CONCENTRATIONS OF Pu 3.0 
Initial H-ion concentration of Czapek's solution 
Pa 3.0 Pa 4.5 
Cc. N/50 KMnO, Ce. N/50 KMnO, 
Pn of P H of : 
buffer buffer 
sol.* Amylase}  sol.* 
Total | Control activity 
Amylase 
Total | Control activity 
Extracellular 
amylase 
-Ó 
_ 
ll adil anal NN 
eee 
BOSSOMNNN| ROSSOW ww 
pi pó ji 
ora OD 0 [o eee IT ET 
OOcoOuw»RWnoolÁnooo»r»roo 
— — 
woooecocoeocoouwnuw 
Intracellular 
amylase 
_ 
_ 
ee comm et OO oo) 
ROSSOOHS|NSCSOSONS 
—- 
m 
SS Oe al a d Le edi eia 
ONWNNANNNIDOCON OA 
oO 000 OO wylo nanan - 0o 00 en 
a w Q2 Q2 Q9 Q2 Q2 C2 M m m e Em E m I 
O00 0 O0 c N N| an iR Oo O o o b an 
— 
p= 
OAAaAtanrtpaARRRAONS 
NNNNNNNNIOCSOSOSSSSOS 
COO ovo is m Ot |o RR OY PS PR 
cooonuneouloooooocutuv 
ren 
* H-ion concentration of the buffer solution in which enzyme activity was meas- 
ured. 
f Distilled water was substituted for the buffer solution. 
