[Vor. 8 
86 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
pared, it is evident that the reaction of the medium seemed to 
have no influence upon the resulting properties as determined 
by the buffer solution. It must be remembered, however, that 
the H-ion concentration of the nutrient solution had, in most of 
the series, been changed during the growth of the fungus. The 
greatest difference, asseen 
l6 in table r, was in Series 1 
and 5 with Fusarium 
where the final reaction 
was Pa 7.2 and 9.2 re- 
spectively. This varia- 
tion might not have been 
decided enough to pro- 
duce a change in the pro- 
cesses within the fungus. 
The results might have 
12 
d been very different if it 
= 3 had been possible to keep 
X the reaction constant. 
x However, although the 
g.s P, 9.2 series of Fusarium 
did not change during the 
experiment and the more 
T alkaline cultures of Colle- 
totrichum were shifted 
2 very slightly, the enzyme 
produced under these con- 
ditions was similar to the 
one produced in the cul- 
" í x " 7 T" 4 " tures which at the be- 
requies etna spon? ging vero Py 80 ox 
totrichum Gossypii grown in Czapek’s solution 44.5. Further, it isim- 
of Pu7.0. possible to say what the 
reaction within the cell 
of the fungus has been during the secretion, but it is significant 
that the enzyme which had been excreted into the culture solution 
retained the properties of the enzyme in the mycelium. Again, 
Euler and Emberg (19) have shown that the enzyme for- 
mation by yeast cells could be modified by the adaptation of the 
