(Vor. 8 
88 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
still it may be noted that the maximum activity relations as 
determined by these experiments were different from those 
obtained by Sherman and his associates (15—17) and by Adler 
(16). This may be due to the fact that the ions other than the 
H ions in the solutions used to determine activity exerted an 
influence. Itisvery likely, 
l6 on the other hand, that 
the time and temperature 
of incubation had some 
influence, since Sörensen 
showed that the H-ion 
concentration at which 
maximum activity was 
produced, in the case of 
invertase, depended upon 
the two above factors and 
the shift seemed to be 
toward neutrality with 
continued incubation. 
Since the amounts of 
the culture solution re- 
maining in the flasksafter 
growth of the funguswere 
the same in all cases and 
the dry weights varied 
very slightly, a summa- 
tion of the activities of 
the intra- and extracell- 
ular enzymescan betaken 
to denote relativeenzyme 
Fig. 9. Action of extracellular (——) and accumulation. A relation 
iesdbdl: (---) amylase produced by Colle- hich . ith 
totrichum Gossypii grown in Czapek’s solution que : varied with the 
of Px9.2. organism seemed to exist 
between the H-ion con- 
centration of the medium and the accumulation of the enzyme. 
If the maximum activity, which is about P, 6.0 in the fore- 
going series, is taken as an index of the amount of amylase pres- 
ent, excretion into the culture solution was greatest for Fusa- 
rium in natural Czapek's solution, for Penicillium in the culture 
ce ^o A/nQ, 
Pu 
