1921] 
ARMSTRONG—SULPHUR NUTRITION IN THE FUNGI, THIOSULPHATE 243 
I contained the following amounts of the salts per 50-cc. culture: 
Na,8,0, M/10, 8.1 cc.; KH,PO, M/6, 1.1cc.; ENO, MA, 3.9 
cc.; NH,Cl MA, 3.7 e MgCl, M/60, 2.9 cc.; FeCl, M/1000, 
.5 cc.; dextrose M/1, 28 cc. as the source of carbon in experi- 
ment 1; sucrose M/1, 2.8 ec. as the source of carbon in experiment 
2. It will be observed from table 1 that where equi-molecular 
volumes of dextrose and sucrose are employed differences in 
TABLEI 
GROWTH AND RELATIONS OF CERTAIN FUNGI ON MEDIA CONTAINING 
SODIUM THIOSULPHATE. TIME INTERVAL OF CULTURES, 7 WEEK 
Experiment Experiment 2 
.2 per cent Na4j3:0; em dextrose| .2 per cent Na2S,0; and sucrose 
Fungus A. nig. | P. gl. | B. cin. |Check| A. nig. | P. gl. | B. cin. | Check 
No. cultures 2 2 2 1 3 1 2 1 
Dry wt. (gms.) .0941, .1351| .1117 .1722| .1800| .2077 
Ce. N/100 I 5.2 13.9 {12.8 15.6 | 1.0 |13.5  |11.1 15.8 
% NazS:0; 
decomposed | 66.6 10.9 18.0 93.6 14.5  |29.7 
2 3 4 2 3 2 
Sulphates + + 4. — i+ + + — 
Sporulation 3 1 | 4 3 4 3 
Cc. N/10 KOH .9 | .9 | 1.3 3 
Ce. N/10 H;S0, Al | al A y! 
growth have occurred which may be due to sucrose as a better 
source of carbon, or to the greater total quantity of carbon sup- 
plied. These organisms are able to use the thiosulphate with the 
production of very noticeable quantities of H,S and sulphates. 
Using phenolphthalein as an indicator, a production of acidity is 
indicated for Aspergillus, while Penicillium and Botrytis produce 
an alkalinity of the solution. 
Two different nutrient solutions as employed by Kossowicz 
and Loew were used in experiments 3 and 4. All salt concentra- 
tions are given for 50-cc. cultures. The solution for experiment 
3 was as follows: Na,S,0, M/1, 1.2 cc.; KH,PO, M/60, 2.2 cc.; 
(NH4,HPO, M/6, 2.2 cc.; KNO; MA, 4.5 cc.; NH,NO, M7, 
1.2 cc.; MgCl, M/60, 2.0 cc.; CaCO, M/100, 1.0 cc.; FeCl, 
M/1000, 1.0 cc.; dextrose M/1, 6.9 cc. 
The solution for experiment 4 was as follows: Na,3,0, M/1, 
