1921] 
ARMSTRONG—SULPHUR NUTRITION IN THE FUNGI, THIOSULPHATE 247 
periment 9 the KCNS and MgCl, were omitted and the mag- 
nesium and sulphur were supplied in 1 cc. of M/1 MgSO, The 
potassium and chlorine content were kept practically the same 
by adding 1 cc. of M/1 KCl. Both growth and fructification 
have been greatly increased where sulphur is supplied as MgSO, 
instead of KCNS. 
TABLE V 
habi CERTAIN FUNGI ON MEDIA CONTAINING POTASSIUM THIOCY- 
AND AMMONIUM THIOCYANATE. TIME INTERVAL 
OF CULTURES, 4 WEEKS 
Experiment 10 Experiment 11 
1 per cent KCNS 1 per cent NH,CNS 
Fungus A. nig. | P. gl. | B. cin. | Check | A. nig. | P. gl. | B. cin. | Check 
No. eultures 2 2 2 2 2 2 
Dry wt. (gms.) | .1313 | .0159 | .0288 .0445 | .0125 | .0250 
H:S 1 3 0 t 1 3 0 
Sporulation 0 0 0 0 0 0 
Nutrient solutions as employed by Kossowicz and Gróller were 
used and are, for experiment 10, KONS 2/M, 2.5 ec.; KNO; M/1, 
2.4 cc.; NH,NO, M/1, 3.1 ec.; KH,PO, M/6, 2.2 cc.; MgSO, M/3, 
3.0 cc.; dextrose M/1, 6.9 cc. The salts in the solution for ex- 
periment 11 were NH,CNS M/1, 6.6 cc.; KH,PO, M/6, 2.2 cc.; 
MgCl, M/60, 7.4 ec.; CaCO, M/100, 1.0 cc.; FeCl, M/1000, 1.0 
cc.; dextrose M/1, 5.6 cc. In the solutions employed, 1 per cent 
of both KCNS and NH,CNS strongly inhibits the growth of the 
3 organisms. 
The nutrient solution for the experiments 12, 14, and 15 of 
tables vı and vir was the same except for the source of sulphur. 
The constituents of the solution were NH,NO, M/1, 6.2 cc.; 
KH,PO, M/1, 2.5 cc.; MgCl, M/1, 1.0 cc.; FeCl, M/1000, .5 
ec.; dextrose M/1, 6.9 cc. The sulphur compounds were added 
in such quantities that the sulphur content of the solutions in the 
three experiments mentioned would be the same. Twenty-five 
ec. of M/50 K,S,0, were added per flask in experiment 12, 1 cc. 
of M/1 KSH in experiment 14, and 1 cc. of M/1 MnSQ, in ex- 
periment 15. The solution to which equivalent amounts of 
