[Vor. 8 
274 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
solution employed in experiment 3 in which Aspergillus evidently 
caused the production of a tetrathionate, there was no apparent 
separation of sulphur, while in several other cultures where the 
separation occurred no tetrathionate could be detected. 
Ory wt 
cgms. 
Py 
p d 
A AP 
100 | MED TU N 2 
Ll L. 
' N " 
/ 
L | 
ff 
(0) 4 8 12 16 20 
Da ys 
Fig. 19. Aspergillus niger on solution of initial Pg 5.5 containing 
magnesium sulphate. 
The tips of many hyphae contained globules of varying sizes 
after a growth period of 21 days in the 5 and 10 per cent solutions 
of thiosulphate. A few days after the end of this period, some of 
the globules were found crystallizing in the hyphae in the shape 
of double pyramids as described by Molisch (13) and Miyoshi 
