[Vor. 8 
208 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
pected, fluctuations sometimes apparently erratic and seemingly 
unexplainable occurred occasionally. In such cases, the experi- 
ments were always duplicated and frequently triplicated. 
The curves are developed from the percentage averages, as 
indicated, each curve representing the final reading of the ger- 
mination quantities of a particular organism at a definite 
temperature in a certain medium. The percentages of spore 
germination are plotted as ordinates and the hydrogen-ion 
concentration of the solutions as abscissae. Curves are shown 
giving germination percentages at each temperature and with 
each medium. In addition an assembled graph is presented, 
this being made by averaging the germination percentages of 
the three temperatures with each of the media. 
|^ < AN 
\ 
^ 
3 
~ 
ae 
è 
" 
Percentage of GSerminehen 
3 
Cd 
Km - 
f 
A 
i4 
v 
> 
Fig. 5. Botrytis cinerea in sugar beet decoction. 
In examining the experimental results, it must be borne in 
mind that perfect germination is not to be expected with these 
fungi in water or in solutions containing mannite and the acid 
or the alkali as the only nutrients. Dextrose, in fact, would 
have yielded higher germination percentages than did mannite, 
but it is not certain that it would remain stable with the treat- 
ment given. 
Of the various liquid media employed in this study, the sugar 
beet decoction has undoubtedly furnished the most interesting 
data. Germination obtained with the acid cultures has not 
