1921] 
WEBB—GERMINATION OF SPORES OF CERTAIN FUNGI 313 
percentages are relatively low in “water HCl or KOH”, but 
the expansion in range beyond that offered by the other culture 
solutions—with the exception, of course, of beet decoction— 
would also tend to indicate a change in reaction during germina- 
tion. In view of these facts, it seemed advisable to conduct a 
few simple experiments which might throw light on this problem. 
Inasmuch as A. niger and B. cinerea furnished such widely 
different results in solutions of mannite and beet decoction, these 
two organisms were selected: for a study of the first aspect men- 
tioned, and the extremely stimulating effect of beet decoction 
upon germination of these spores is shown by the data accu- 
mulated from experiments involving mixtures of the mannite 
and beet decoction solutions in various proportions, as presented 
in the tables for the respective organisms. 
The same organisms, together with Fusarium sp., were em- . 
ployed in studying the second possibility, that is, changes in 
the reaction of the medium during germination. These data 
appear in tables x, x1, and xu, and will be considered subse- 
quently in the discussion. 
TABLE X 
BOTRYTIS CINEREA. INITIAL AND FINAL HYDROGEN-ION CON- 
CENTRATIONS DURING GERMINATION FOR 20 HOURS AT 24° C 
Hydrogen-ion concentration, Px Media 
Initial | 1.6| 2.0] 2.5] 3.0/4.0/5.3|6.217.017.518.118.619.6 = 
Final | 1.6] 2.0/ 2.5] 3.0/4.0 5.3 7.0|7.5/8.0|8.2|9.1 i 
Control | 1.6|2.0|2.5| 3.0]4.0/5.3]6.2|7.017.5|8.1]8.2/9.0 S 
Initial |1.2|1.8| 2.1] 2.8|3.4| 3.6 | 5.0 | 6.1 | 6.7 |7.2|8.0|8.9|9.6| 3 
Final 1.2| 1.8| 2.1 | 2.8|3.4| 3.6 | 5.0 | 6.1 | 6.7 | 7.2 |7.8|8.2|9.1| 8 
Control | 121 1.8|2.1|2.8]3.4/3.6/5.0/6.1]6.7|7.2|7.8]82|9.0|] 5 
Initial |1.8|2.4|2.7|3.1|3.6 4.2 | 5.0 | 5.8 | 6.5 | 6.8 | 8.0 | 8.6 | 8.9 esa 
Final | 1.8| 2.3) 3.6| 3.8|3.8| 4.7 | 4.8 | 5.5 | 5.7 | 6.4 | 6.7 | 7.9 | 8.6 $88 
Control | 1.8/ 2.4| 2.7 | 3.2|3.8| 4.4 | 5.0 | 5.8 | 6.6 | 7.0 | 8.1 |8.3|8.8| > 
Initial | 1.6] 2.0| 2.4] 3.0|4.0| 5.5 |6.2|7.0|7.7|8.1|8.21|8.6 sok 
Fin 1.6| 2.0] 2.4| 3.0/4.115.516.2] 7.0] 7.6|8.1|8.218.6 sus 
Control | 1.6] 2.0|2.4| 3.0/4.1] 5.5 |6.2|7.0|7.7/8.118.2| 8.6 n4 
Initial | 1.4|2.0|2.4| 2.9|4.0| 5.1 | 5.5 | 6.3 | 6.6 | 6.9 | 6.9 | 7.1 | 8.6 |, 
Final | 1.4| 2.0| 2.4| 3.2|6.0| 6.7 | 6.8 | 6.7 | 6.9 | 7.1] 7.1/7.1 | 7.6 |Š9Q 
Control | 1.4 | 2.0] 2.4 | 2.9 |4.6| 6.2 | 6.6 | 6.7 | 6.9 | 7.1 [7.2 |7.3 | 7.7 P^ & 
