1921) 
329 
WEBB— GERMINATION OF SPORES OF CERTAIN FUNGI 
“Water H,PO, and NaOH" followed by solutions of mannite, 
undoubtedly gives the lowest percentages and the smallest range - 
of germination. The conditions in the two solutions are very 
comparable; that is, the individual cultures contain equal 
quantities of H,PO, and increasing quantities of NaOH, result- 
ing therefore in the formation of NaH,PO, and Na,HPO, at 
Py 4.5 and Py 9.2 respectively. No Na ions are contained in 
the most acid culture and these ions increase in number with 
decrease in H-ion concentration. It is impossible, however, to 
100 "1 
P. EV 
à ff 
uy x T 
i V1 
è U 
E 4n VAM 
i Ty 23% 
/ 39% 
: / ‘a 
Ns , Fi 
ies I 
S 1 l 
$ E 
E E 
| P» L 
d Ls 
4 
2 3 4 s 6 7 & 9 /o 
D 
Fig. 31. Fusarium sp. in 2 per cent bacto-peptone solution. 
vary the H-ion concentration without varying the concentration 
of some other ion or ions at the same time, even though such 
variations may be insignificant. 
Solutions of bacto-peptone give very similar results to solutions 
of mannite, except that germination is better, that the range is 
slightly more extended on the alkaline side, and that maximum 
germination frequently occurs in zones rather than at definite 
points. The reaction of the peptone solutions, as well as Czapek's, 
beet decoction, and “water HCl or KOH," is varied on the acid 
side by additions of HCl and on the alkaline side by additions 
of KOH. Disregarding the variation in H-ion concentration, 
the acid cultures thus contain increasing amounts of Cl ions 
and the alkaline cultures increasing amounts of K ions. 
