[Vol. 9 



370 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



Corn meal agar slants. — Growth not as good as on lima-bean 

 agar (ingredients in the same proportion as in lima-bean agar) ; 

 it was filiform, quite the same whitish color as that possessed 

 by the medium itself, and somewhat raised and glistening. 



Whey agar slants. 1 — Growth at 30° C, medium testing Ph 5.4, 

 was good; it was raised, filiform, ivory-yellow in color (see Ridg- 

 way, Color Standards, 1912), glistening, margin slightly undu- 

 lating or entire, odor none, consistency slimy in the moist part 

 at the base of the slant, and butyrous, somewhat sticky, in the 



ppeared 



tendency to produce sm 



matrix. Surrounding the smear there was at first a colorless zone 

 followed by a white precipitate, but by the fourth day the color- 

 less area disappeared, due to an extension of growth at the margin 

 overgrowing the area. The margin finally appeared markedly 

 white in contrast to the interior, this whiteness being due to the 

 white precipitate which had previously been formed when the 



smear 



removing the bacterial 



it was seen that the white portion coincided with the region 

 where precipitate had been formed.) Growth on whey agar 







.0 and P H 7.2 was slightly better than on P H 5 

 medium, but there was no colorless region and no precipitat 

 On Pn J).0 whey agar there was no growth. 



Uschinsky's solution. — Very slight growth in 3 days kept at 3( 

 C, medium testing P n 7.6; at the end of 10 days there was a 

 marked ring with a faint surface growth and the fluid was ren- 

 dered markedly viscid. 



Fermi 's solution. — Noticeable surface growth in 3 days which 

 broke up into very fine particles on agitation and left a ring 

 around the tube; there was but slight clouding. At the end of 

 7 days there was a very marked surface growth and a dense 

 clouding in the upper two-thirds of the medium. 



Cohn's solution — No growth. 



Potato cylinders. — Growth at 25-30° C. good, glistening, buck- 

 thorn-brown (Ridgway), sticky, slightly viscid. After growth of 

 1 month the cylinders were macerated without difficulty, 10 cc. 

 of distilled water was added to each tube, and, in addition, 1 cc. 

 of potassium iodide. A reddish purple color immediately devel- 

 oped which upon standing for several hours entirely disappeared, 



'Made up according to the formula given by Miss Bryan (II, '21, p. 149) except 

 that Bacto-golatin was used instead of Nelson's photographic gelatin, No. 1. 



