32 



[Vol. 10 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



With 



(No. 191 



ated by 



stabbing gelatin plugs grew and responded almost identically 

 as may be seen by glancing at table in. 



Milk. — Fresh milk (+20) incubated at 28 C. is promptly coagu- 

 lated. The amount of acid developed by the organism increases 

 steadily for 18 days at least. Peptonization slow, begins in 2-3 days. 

 In similar cultures to which litmus was added, a marked bleaching of 

 the indicator took place by the end of the eighth day, as a rule. The 

 addition of 2 per cent (by volume) of a saturated solution of Merck's 

 purified litmus had no noticeable inhibitory effect upon the growth of 

 the strains of the blackleg bacillus tested. 



It may be noted that considerable difference appears in the 

 descriptions published by earlier authors as to the cultural fea- 

 tures and physiology of the blackleg bacillus when grown in milk. 

 The writer failed to discover any real differences among the 

 strains cultivated by him except in the case of strain No. 191 

 which, as has appeared heretofore, is non-pathogenic and other- 

 wise quite different in its cultural features and physiology (see 

 table m). 



Uschinsky's solution. — Growth copious; fluid not viscid. 



In this solution in which the nitrogen is supplied by organic 

 compounds (see p. 28) all strains cultivated developed simi- 

 larly except No. 191, which grew more copiously than the others 

 with the development of a pellicle upon the surface of the me- 

 dium. 



TABLE III 



CULTURAL FEATURES AND PHYSIOLOGY 



GELATIN STAB* 



REACTION 



INCUBATED AT 20° C 



Culture 



160.1 



170.3 



180.2 



183 2 



187B.1 



191 



194. 



195. 



196. 



197. 



198. 



201 



Growth 



Line of 

 puncture 



Liquefaction 



Type 



Begins 



Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 

 Best 



at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 

 at top 



Fil.-beaded 



Fil.-beaded 



Fil.-beaded 



Fil.-beaded 



Fil.-beaded 



Filiform 



Filiform 



Filiform 



Fil.-beaded 



Fil.-beaded 



Fil.-beaded 



Fil.-beaded 



Strati.-infund. 



Stratiform 



Napi. -strati.... 

 Napi.-strati.... 

 Crater.-strati. 



None 



Strati.-infund. 

 Strati.-infund. 

 Napi.-strati. ... 

 Crater.-strati.. 

 Napi.-strati ... 

 Crater.-strati.. 



avs 

 2 



I 



I 



1 

 2 



1 

 1 

 1 

 2 

 1 

 2 



Complete 



d a va 

 22 

 22 

 22 

 22 



99 



22 

 22 

 22 

 22 

 22 

 22 



