64 



[Vol. 10 



GARDE 



II 



Quantitative determinations of carbohydrate utilization show 



that Bacillus atrost pticus cannot hydrolyze potato starch or dex- 

 trin. 



This organism, however, can utilize the saccharides, glucose, 

 fructose, galactose, sucrose, lactose, and maltose. 



The several strains (9) of B. atroscpticus investigated were 



presented 



their ability to hydrolyze and 



Bacillus coli can utilize the carbohydrates, lactose, maltose, 

 dextrin, and potato starch, as well as glucose, fructose, and 

 galactose. The strain investigated did not hydrolyze; sucrose, 

 nor was the amount of the original of this sugar reduced. 



Bacillus vulgatus can consume the saccharides, sucrose, mal- 

 tose, dextrin, and potato starch, as well as glucose, fructose, and 



galactose. The strain investigated could not hydrolyze nor con- 

 sume lactose. 



Under the conditions of the experiments a carbohydrate con- 

 centration of 0.25 per cent, 0.4 per cent, and 0.5 per cent would 

 have furnished an ample supply for Bacillus atrosepticus, B. coli, 

 and B. vulgatus, respectively. 



The yeast species investigated utilized only glucose and fruc- 

 tose, of the carbohydrates presented. Both these saccharides 

 were completely removed by this organism in less than 6 days, 

 under the conditions of the experiment. 



The saccharides, sucrose, lactose, maltose, dextrin, and po- 

 tato starch, were not hydrolyzed appreciably if sterilized in the 

 autoclave when dissolved in the Dunham's solution employed. 



The disaccharides, sucrose, lactose, and maltose, were hydro- 

 lyzed with about equal rapidity in both the autoclave and the 

 steamer when a very small amount of a mineral acid (0.4 per 



tion. 



HC1) was added to "adjust" the Dunh 



Acknowledgments 



The author wishes to express his most sincere appreciation of 

 the cooperation and assistance accorded by many colleagues and 

 workers. Especial thanks are due to Dr. B. M. Duggar, Dr. 



