

122 



[Vol. 10 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



mber have been continued from the beginning of the work 



the, 



August, 1922, did 

 viouslv isolated st 



Strain 17, isolated from a diseased pod 



differ 



from 



strains, but it was carried in culture because 

 found to produce stylospores more abundantlv than 



isolated form 



In September the writer tempo- 

 rarily moved to St. Louis, Mis- 

 souri, taking with him cultures of 



several strains, 

 vember the orii 



By 



N 



< 



O 



f\ 



<• 



'•X 





^' 



\J. 



» m 



v.- 



i 



certain subcultures of strain 17, 

 were observed to have formed 

 mature perithecia and asco- 

 spores. Examination of 5 other 



U 



%j 



i 



**\m '-■ 



f^ 



Fig. 5. 



strains, Nos. 7, 11, 14, 19, and 

 20, showed that perithecia had 

 not yet developed in any of these. 

 In March, however, it was dis- 

 covered that strain 1 8, which had 



Ai>ci of soybean fungus, con- Deen overlooked in the previous 

 taining ascospores. examination, had also formed 



ascocarps in every way simi- 

 those of No. 17. Strains 17, 18, 19, and 20 were isolated 



the same time but from different plants, 17 and 



from diseased pods and 19 and 20 from 



the 



of diseased stems 



Transfers from ascosi 

 similar to those from 



off 



manner 



lm 



strains. 



mature in the course of 



cnidia bearin 



days, but the 



are somewhat less abundant than in cultures of imperfect forms. 

 The pycnidia persist for an indefinite period and are then rather 

 suddenly replaced by perithecia. These fruit bodies may appear 

 in culture in as short a time as 41 days after inoculation when 

 kept at laboratory temperatures during the months of February 

 and March. 



In cultures 



bean 



the most favorable 



substratum found by the writer, the perithecia are black in 



and 



rendered 



by the tendency 



form 



