124 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



[Vol. 10 



droplets diminish in size and vacuoles of various dimensions 



the germinatin 



then in the 



erm 



tube (fig. 



Irregularly shaped black stromatic or pseudo-pycnidial bodies 

 are formed in cultures of this fungus. On agar media in plates 

 and tubes they occur at various p laces over the surface, nar- 



the glass sides of the 



When the fun 



ao* 



Fig. l>. Ascoaporea of soybean 



fungus. 



Fig. 7. Ascospores of soybean fungus 



germinating. 



is grown on sterile soybean or sweet clover stems in tubes in the 

 bottoms of which cotton or blotting paper has been placed to 

 hold moisture, the mycelium grows down into this material and 

 forms flat, round, or irregularly shaped stromatic bodies of various 

 sizes. These possess a dense black cortical layer having a thick- 

 ness up to .4 mm. and enclosing a white or dilute brown aggrega- 



hyphae closely interwoven with the cellulose 



In culture, these bodies have developed 



tion of fungous 

 fibers of the substratum 



directly on stems of Melilotus alba and were then about the size 

 and shape of a small radish seed. Hand sections show that these 

 bodies possess a cortex of 3 or 4 rows of very dark brown or black 



cells surrounding a pseudo-parenchymatic tissue of thinner- walled, 

 dilute brown to hyaline elements, the whole resembling a sclero- 



tium 



the 



out, one to several pycnidial beaks, which sometimes exude 



feebly, may develop on these stromata 



perfect 



strains develop these bodies in a period of time corresponding to 

 that required by perfect strains to form mature perithecia. 



They resemble in many ways the fertile 



perf 



