19231 



LEHMAN — POD AND STEM BLIGHT OF SOYBEAN 129 



short or none; wall thin beneath, thick sclerotial above, outer 

 layers black, inner layers dilute brown; stroma diffuse or lacking; 

 sporophores hyaline, simple, slender, tapering, 1% to 3 times 

 the length of the spores; pycnospores hyaline, continuous, 



i 



usually 2-guttulate, oblong, often fusiform, seldom curved, 6.27- 

 7.15 X 2.18-2.31 [x; stylospores seldom present, hyaline, slender, 

 curved or hooked. Perithecia spherical or mutually compressed 

 laterally, simple, immersed in black stromata, 145-348 X 116— 

 318 p; beak very long, slender, tapering, 1.5 mm. X 40-60 (t, 

 black; wall definite, outer layer black, inner layer hyaline; asci 

 sessile, elongate, clavate, thin-walled, 8-spored, 37.2-50.2 X 

 7.2-12.2 (x (average 44.9 X 8.3 n), apex thickened and pierced 

 by a narrow pore; ascospores hyaline, elongate-elliptical, 1-septate, 

 9.6-12.4 X 2.4-4.2 p (average 11.4 X 3.5 ti), slightly or not at all 

 constricted at the septum, possessing 2-4 guttulae. Perithecia 

 found only in culture. 



Parasitic on Soja max (L.) Piper in North Carolina. 



Isolations 



Isolations have been made from diseased stems, petioles, pods, 

 seeds, cotyledons, and seedling hypocotyls. Diseased plants 

 bearing pods and seeds were brought into the laboratory and 

 stored in a screen-covered cage. This was used as material 

 for making isolations at various times during the following 

 winter months. Bundles of diseased plants were also stored 

 out of doors. A partial record of the isolations with the date 

 and source is given below. 



1. Made on August 20, 1920, by Dr. F. A. Wolf, from a diseased 

 stem. A loopful of pycnospores suspended in sterile water was 

 spread with a waving motion of the needle over the surface of a 

 hardened agar plate. Individual colonies were sufficiently well 

 separated near the end of the stroke to be easily picked from 

 the plate. Growth typical for this fungus on nutrient agar, 

 such as will be described below, developed in a few days. 



3. Made September 20, 1920. (a) Pycnospores from pycnidia 

 on a diseased pod were spread on the surfaces of hardened agar 

 plates, (b) The pods were opened carefully and the seeds 

 found to be covered with a white mycelial web. By use of a 



