[Vol. 10 



132 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



a fungus which produced many pycnidia characteristic of the 

 Phomopsis stage of D. Sojae on sterile soybean stems This 

 strain has been used in subsequent cultural studies and inocula- 

 tion work. 



9. Made February 18, 1921. Five seeds which were either 



slightly discolored or both discolored and wrinkled were selected 

 from a lot shelled from a plant which bore segregated, pycnidial 

 areas on the stem, but none on the pods. After disinfection, 

 the seeds were put into large test-tubes containing blotting-paper 

 moistened with Shive's 3-salt nutrient solution. Two seeds 

 which failed to germinate soon became covered with a dense 

 growth of fungus resembling that of D. Sojae in every way. 

 This was not further tested. Of the 3 seedlings which germinated 

 1 was killed when the hypocotyl had attained a length of 1 V^ inches 

 by growth of a fungus downward from the seed-coat and coty- 

 ledons on to the hypocotyl. Pycnidia of D. Sojae developed on 

 the hypocotyl and seed-coat. Healthy seedlings reach a height 

 of 5 or (> inches before dying when grown under these conditions. 



10. Made March 12, 1921. Five slightly wrinkled and dis- 

 colored seeds selected from plants in which the stem and pods 

 bore scattered pycnidia were sterilized and put into large test- 

 tubes containing blotting-paper moistened with the 3-salt 

 nutrient solution. At the end of the seventeenth day the seedling 

 from the only seed which germinated had been killed after 

 attaining a height of 2% inches by growth of a fungus from the 

 cotyledons to the hypocotyl. Pycnidia of D. Sojae formed on the 



stem below the cotyledons. 



12. Made April 23, 1921. This strain was isolated by making 

 a poured plate of pycnospores from a pycnidium on a diseased 

 seedling. The seedling grew from an infected seed which after 

 being sterilized was germinated in a large test-tube containing 

 sterile moist blotting-paper. 



14. Made February 15, 1922. Five seeds were taken from a 

 diseased plant which had been collected on September 1, 1920, 

 and stored in a wire cage in the laboratory. These seeds were 

 disinfected by the method recommended by Norton and placed 

 on moist sterile blotting-paper in large test-tubes. One seed 

 did not germinate but gave rise to a fungus which formed stro- 



