[Vol. 10 



136 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



The pod-blight organism was reisolated from each of these plants. 

 A third plant bearing half-grown pods was inoculated on the 

 same date by inserting mycelium of strain 7 into the wall of the 

 pod. After 5 days under the bell jar infection was apparent on 

 inoculated pods. Around the point of inoculation there was 

 plainly visible a circular area of darkened tissue, 8 mm. in diam- 

 eter, bearing whitish tufts of mycelium on the surface. 



On May 28, 2 plants 18 inches high and bearing several pods 

 each were inoculated by inserting pycnidia of strain 7 into the pod 

 walls of one plant and by laying the inoculum on the surface 

 of the pods of the other. The plants were then covered with a 

 bell jar. On June 7, infection was apparent on 2 of the wounded 

 pods. The causal organism was isolated and found to be identi- 

 cal with that in the original cultures. On July 12, the 4 remaining 

 pods of this plant were brown and apparently mature. However, 

 upon being put into moist chambers, all 4 developed pycnidia. 

 On June 29, 2 of the pods inoculated by placing pycnidia on the 

 unwounded surfaces gave evidence of infection as shown by 

 failure to fill out and by discoloration. The causal organism 

 was reisolated from the interior of these pods. A few days 

 later other inoculated pods on the same plant developed surface 

 pycnidia. In all, 4 of the 6 inoculated pods of this plant became 

 infected. 



On July 7, Pots 13 and 16, each containing 3 plants, were 

 inoculated with strain 12. In each jar one plant was inoculated 

 by inserting mycelium into the stem and the wall of the pods, a 

 second plant by laying inoculum on unwounded pods, and the 

 third by rubbing a spore suspension on wounded pods. Pot 13 

 was covered with a bell jar for 4 days and Pot 16 was left un- 

 covered. By July 22, 3 pods on plants of Pot 13 inoculated by 

 inserting mycelium, and 2 pods on a plant inoculated with a 

 spore suspension, had become infected. In Pot 16, infection was 

 evident on 5 pods of the plant inoculated by inserting mycelium 

 into the wall of the pod. Cultures reisolated from one of these 

 pods did not differ from those of the strain used for inoculation. 



On June 25, inoculations were made as follows: Pot 21 con- 

 tained 1 plant with 18 pods varying in length from 1 to 2% 

 inches, the oldest being apparently full-size but still green. 



