[Vol. 10 



244 ANNALS OF THE MISSOURI BOTANICAL GARDEN 



carpel walls which were completely digested. On the surface there 

 was a dark central area surrounded by a brown area shading to pink 

 at the outer edges. This fungus also attacked the damp cotton upon 

 which the sterilized lemons had been placed and digested it rapidly 

 in spite of the fact that it had only been wet with sterile, distilled water. 



Cultures of these fungi were sent to Mrs. Patterson, of the 

 Office of Pathological Collections, Bureau of Plant Industry, and 

 examined there by Miss Jenkins, who was of the opinion that 

 they might represent different species. Subsequently it was 

 learned that the various California species of AUemaria were 

 being studied at the Citrus Experiment Station, Riverside, so 

 no further identification work was carried out. 



Phomopsis Citri Fawcett. — This culture was furnished by Dr. 



isolated from 



Fawcett, while later cultures were easily 

 oranges from Florida. The fungus did not grow well in most 

 liquid media as it grew in the solution instead of forming a mat 

 on the surface. Due to this habit of growth, it probably obtained 

 insufficient oxygen in liquid culture, this being indicated by the 



of 



5 



of 



large amount of the rotting of oranges shipped from the Florida 

 district. It did not grow at high acidity nor did it rot lemons. 



Sclcrotinia Libert iana Fuckel. — Selerotia of this 



■ 



B 



obtained from Professor Home of the University of California 

 Agricultural Experiment Station. The selerotia germinated 

 readily, but this fungus was found to be very erratic in culture. 

 In the presence of peptone it usually grew well in liquid media, 

 but with inorganic nitrogen the growth tended to be scant and th 

 formation of selerotia occurred very early in the period of growth. 

 This is the cause of the so-called "cottony rot" of lemons which 

 occurs chiefly in the packed crates of fruit. 



In order to facilitate the making of tables and the discussion 

 of results the organisms used will usually be referred to by number 

 as follows: 



2. Penicillium stoloniferum 7. AUemaria Citri 



3. Penicillium sp. 9. Aspergillus sp. 



4. Diplodia natalenris 11. Sclerotinia Libertiana 



5. Phomopsis Citri 14. AUemaria sp. 



6. Oospora Citri- aurantii 16. Penicillium digitatum. 



Cultural methods. — In carrying out the succeeding work the 



