[Vol. 10 



266 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



Penicillium sp. — In fig. 2 are given the data for solution 1 and 

 in fig. 3 the data for solution 2. It is to be noted in connection 

 with these curves that the time elapsing between inoculation and 

 the beginning of chemical determinations was 5 days in the case 

 of solution 1, but in the case of solution 2 it was 10 days. Taking 



Irvsr. 



CultZ SJ'n. I 



i+ 



Fig. 2. Penicillium sp. in solution 1 



this into consideration there is to be noted a general deterrent 



effect upon the starting of growth in solution 2; this was probably 



due to the fact that the P H of the solution was unfavorable for 



growth and that this situation was overcome much more readily 



in the slightly buffered sugar solution than in the heavily buffered 

 solution 2. 



maximum weight of mat attained 

 a twice that attained in solution 1 . 



Apparently in solution 



