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[Vol. 10 



ANNALS OF THE MISSOURI BOTANICAL GARDEN 



of P H for the 2 solutions will be found in fig. 8. For this fungus 

 the solution was made slightly more alkaline than in the pre- 

 ceding work, solution 1 containing 20 cc. of 2 N HiPO« and solu- 

 tion 2, 175 cc. of 2 N KOH per liter. So closely did the duplicate 



Cbyj 3 



Fig. 7. Penicillium stoloniferum in solution 2. 



mats and chemical analyses agree in the case of organisms 3 and 

 9, and so great was the amount of routine required that it was 

 decided to mix the duplicate solutions in each case and run one 

 determination on the mixed solution in all the succeeding work. 

 The mat from solution 2 was about two-fifths heavier than that 

 from solution 1, as was also the case with organism 9, and the 



