334 



[Vol. 10 



ANNALS 



Organism 



Nectria Ipomoeae 



Nectria Ipomoeae 



Nectria Ipomoeae 



Sphaeropsis malorum 



Sphaeropsis malorum 



Sphaeropsis malorum 



Diplodia natalensis 



Diplodia natalensis 



Diplodia natalensis 



TABLE III 



NITROGEN FIXATION 



Days 

 incubation 



14 

 14 

 14 

 14 

 14 



Mgm. N in 

 culture 



34.82 

 35.11 

 34.77 



35.39 

 35.05 



14 



29 



29 



18.09 



14 

 14 



14 



34.94 

 34.82 



- 



34.26 

 35.56 

 34.94 



14 

 14 

 14 



17.98 

 18.09 

 18.38 



29 

 29 



14 



14 



34.65 

 34.71 



36.19 

 35.51 



14 

 14 

 14 



29 

 29 



18.72 

 18.78 

 19.00 



35.34 

 35 . 79 



Mgm. N in 

 check 



34.82 

 34.76 



17.98 



34.82 



34.71 

 34.71 



17.98 

 18.03 



34 71 



35 79 



18 09 

 17 93 



35 79 



The analytical data for the work with Aspergillus niger, 

 Sphaeropsis malorum, and Diplodia natalensis are given in the 

 following tabulations and graphs. The heavy vertical lines of 



the graph 



the days 



hich determinations were 



made. The [h] of the "peptone plus dextrose" medium is seen 

 to increase during the first 3 to 5 days in cultures of all 3 fungi; 

 a comparison of the curves for dry weight and [h] shows that the 

 rise in [h] is proportional to the rapidity and amount of growth 

 of the fungi. For the very fast-growing Aspergillus the maxima 

 of growth of the organism and [h] of the medium are coincident 

 in time; whereas for the other 2 fungi there is a distinct lag of 

 2 to 3 days in the growth maximum. That both these factors 

 are intimately related to the carbohydrate consumption is 



