1923] 









KLOTZ — NITROGEN METABOLISM IN FUNGI 365 



5. Ammonia is the chief nitrogenous product of autolysis and 

 is a waste product of the splitting of the peptone of the media in 

 the absence of another C source. In the presence of dextrose 

 NHi was reassimilated. Disappearance of carbohydrate from 

 the culture medium is synchronous with the beginning of auto- 

 lysis. 



6. In cultures whose H-ion exponent becomes greater than 

 P H 7.0 the loss of N is due to the evolution of NH,. 



7. Conditions necessary to the formation of crystals of 



MgNH4PO«.6H,0 and Mg,(PO«),.4H,0 in the cultures were 

 determined. 



8. The probable causes for the shifting of the H-ion concen- 

 tration of the media are discussed. 



9. Nitrogen of the amino group is readily assimilated by the 

 fungi studied. 



10. Some factors influencing the N content of the fungous mat 

 are the N and C sources of the medium, the length of incubation, 

 rate of growth, and hydrion concentration. 



11. The organisms displayed markedly different physiological 

 relations ; this was indicated by their rates of growth and of sugar 

 consumption, by their utilization and excretion of the several 

 forms of nitrogen, and by the varying nature and extent of H-ion 

 change of the medium. 



The writer here expresses his appreciation to Doctor B. M. 

 Duggar for criticisms and suggestions given throughout the work, 

 and to Doctor George T. Moore for the privileges and facilities 

 of the Missouri Botanical Garden. 



Graduate Laboratory, Missouri Botanical Garden. 



Bibliography 



Association of Official Agricultural Chemists ('21). Official and tentative methods 



of analysis of the Association of Official Agricultural Chemists. 417 pp. 18 f. 

 Washington, 1921. 



Boas, F. ('17). Weitere Untersuchungen tiber die Bildung starkeahnlicher Sub- 

 stanzen bei Schimmelpilzen. Biochem. Zeitschr. 81: 80-86. 1917. 



■ — , ('18). Weitere Untersuchungen uber die Bildung losUcher Starke bei 



Schimmelpilzen mit besonderer Beriicksichtigung der Frage nach der Eiweisa- 

 synthese der Schimmelpilze. Ibid. 86: 110-124. 1918. 



('19). Bermerkungen uber konidienbildende Stoffe bei Pilzen. Ber. d. 



deut. bot. Ges. 37: 57-62. 1919. 



