OPALINID CILIATE INFUSORIANS — METCALF 467 



(data on Australian Anura); Prof. W. B. Benham, of Dunedin, New 

 Zealand (specimens of the very rare Liopelma and data as to its 

 habits and development); Prof. G. E. Gates (Anura from Rangoon, 

 Burma); Prof. Robert Hegner (Anura from the Philippine Islands); 

 Dr. Ergastri Cordero, of Montevideo, and Dr. Carlos Porter, of 

 Santiago de Chile (South American Anura and data); and Prof. E. V. 

 Cowdry (two specimens of the very rare Heleophryne regis). Miss 

 Margaret Cowles (Mrs. Wilson Shaffer), of Johns Hopkins University, 

 has worked through the life history of Opalina virguloidea in tadpoles 

 of Rana sylvatica, as well as helping in the preparation of some of the 

 South American material. I am also indebted to Mrs. Lura Carper, 

 of the zoological laboratory of the Johns Hopkins University, for 

 revising the bibliography, and to Mrs. CaroUne Hutzler Bernstein for 

 copying several drawings of opalinids used. 



For all this assistance from all sources and for the many personal 

 kindnesses accompanying it I take this opportunity to express most 

 grateful appreciation. 



DESCRIPTIONS OF SPECIES AND NEW DATA AS TO HOSTS AND 



DISTRIBUTION » 



Genus PROTOOPALINA Metcalf 



PROTOOPALINA APPENDICULATA Fantham 



Figure 21 



Host: Rana juscigula Dumeril and Bibron, from Johannesburg, 

 South Africa. 



This is a distinct and very 

 interesting species, chiefly 

 because of the marked tail. 

 An elongated, slender, pos- 

 terior end is characteristic 

 of numerous species of Pro- 



toopalina. In all of them the Figube 21— Protoopallna appendkulata Fantham, X 470. 



cilia are long at the anterior (After Fantham) 



end of the body, and they usually diminish in length and in number 

 toward the posterior end, which is free of cilia. See p. 559 for a dis- 

 cussion of the comparative structure of the posterior ends of different 

 species in the several genera. 



Fantham's specimens measured, in microns: Length, 87-136; 

 width, 22-51. Nucleoli are described as 2 and 4 in different indi- 



» The drawings that illustrate this section are generally Incomplete; for example, usually only a few of the 

 cilia are drawn, or but few of the nuclei in the multinucleate species; only few of the lines of cilia are Indi- 

 cated and these only partially. Only enough is shown to give the features used in diagnosis of the species. 

 A pair of dots outside the contour of the body in the drawings when found indicate the limits of the mor 

 phologically anterior end. 



