OPALINID CILIATE INFUSORIANS — METCALF 



479 



FiGTJRK M.—Protoopalina 

 1/unnaneniii, new species: a, X249; 

 6 and c, the nuclei (from a second 

 and a third individual) XIOIO. 



PROTOOPALINA YUNNANENSIS. new specie* 



Figure 34 



Type: U.S.N.M. No. 22626. 



Host: Bombina maxima (Boulenger), from the Province of Yunnan, 

 southwestern China, at the eastern end of the 

 Himalaya highlands. 



In the only specimen of this bell toad that 

 I had for examination (U.S.N.M. No. 86068) 

 four distorted Protoopalinas were found. 

 Figure 34, a, shows the least distorted speci- 

 men, and figure 34, b and c, show single 

 nuclei from pairs in two others. The nucleoli 

 are fragmented in each case. 



Measurements, in microns: Body about 230 

 by 46 ; nucleus (the longer) 18 by 10. 



This species differs from P. luzonensis 

 in having the nuclei near together in 

 the center of the body instead of far 

 apart (one far forward, the other near 

 the middle). They are probably dis- 

 tinct. This is the species referred to, 

 but not described, in my paper on 

 the bell toads and their opalinid parasites (Metcalf, 1928a). 



PROTOOPAUNA YUNNANENSIS CHENI. new sabspecies 

 FiGtIBE 35 



My friend Dr. T. T. Chen sent me sUdes and drawings of Pro- 

 toopalinas from Bombina maxima Boulenger collected in Yunnan, 

 and he has kindly allowed me to include his form in this paper. It 

 is much larger and has larger nuclei than yunnanensis, and in every 

 individual seen the nuclei are united by a thread. 



In each of the other species of Bombina studied — igneus, pachypus, 

 and orientalis — there are foimd two species of Protoopalina. Bombina 

 igneus and B. pachypus, occurring in Europe, each carry (but not 

 in the same individual) P. caudata and P. intestinalis. Bombina 

 orientalis, which is found in an extensive region centering around 

 the base of the Korean Peninsula, carries (also in separate individual 

 hosts) P. macrocaudata and P. orientalis. Protoopalina caudata has 

 6 nucleoU; P. intestinalis and P. macrocaudata have 8; their number 

 is undetermined in P. orientalis, P. yunnanensis, and the form cheni. 



Protoopalina yunnanensis and P. cheni more nearly resemble each 

 other than do either of the other pairs of Protoopalinas in a species 

 of Bombina, and we can indicate this by classing, say, cheni as a 



