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VOL. 87 



subspecies of yunnanensis. It would, however, seem well to know 

 the number of at least the nucleoli in the two Protoopalinas from 

 Bomhina maxima before decidmg more than tentatively as to specific 

 or subspecific divergence between them. If the numbers of the 



3 



Figure Zb— Protoopalina yunnanensis cheni, new subspecies: a, A group of individuals from one infection, 



X 133; 6, an individual X 482. 



nucleoli are different in the two forms it would seem natural to class 

 them as separate species. Among the specimens of the form Dr. 

 Chen has sent me are none that show mitosis, and so nucleolus 

 number has not been determined. 



Bomhina maxima occurs in the probable ancestral home of the 

 Discoglossidae (see Stejneger, 1905), and its Protoopalinas may well 

 be the most archaic of the species infecting the Bombinas. The most 

 probable view of the origin of the Bombinas and their Protoopalinas 



