618 PROCEEDINGS OF THE NATIONAL MUSEUM vol.87 



Bhatia and Gulati, 1927, reported opalinids as follows from India: 

 From Bana tigerina, Opalina coracoidea lahorensis, new subspecies, 

 new host, new locality; from R. cyanophlictis, 0. ranarum, new lo- 

 cality; from R. hexadactyla, 0. lata, new host, new (?) locality; from 

 Bujo melanostidus, Cepedea metcalfi, new species, C. punjabensis, new 

 species, C. sialkoti, new species. 



Metcalf, 1927a, discussed the evolution of the Opalinidae from the 

 standpoint of certain trends (to flatness, to elongation, to posterior 

 pointedness, to delay in division of the body, to delay in completion 

 of mitosis), phenomena so distributed among the subdivisions of the 

 family as to involve either repeated fortuitous appearance of these 

 characters, a thing not to be believed, or trends resident in the germ- 

 plasm. These conditions are compared with similar phenomena in 

 the Ophryoscolecidae and the Salpidae, and the relation of trends to 

 evolution is discussed. 



Tonniges, 1927, described mitosis in Opalina ranarum, bringing it 

 into line with that of other organisms. Eight ''macrochromosomes" 

 [nucleoli] are described, 24 "microchromosomes" [chromosomes]. The 

 "nucleolus" disappears during mitosis [against Metcalf, 1909). Ami- 

 totic division is described and figiu-ed by the author. Figures of 

 mitosis and of direct division, prepared in 1897, are here published 

 for the first time. 



Metcalf, 1927b, points out that Gourvitsch's Opalina elongata is 

 Metcalf 's Cepedea saharana. 



Lavier, 1927, describes four infections of Protoopalina nyanza from 

 a lizard, Varanus niloticus, from the shores of Lake Victoria Nyanza. 

 The description is quoted in the present paper. 



Sokolska, 1927, reported for Opalina ranarum the Golgi apparatus ' 

 and mitochondria as disk-shaped bodies strewn through the cytoplasm, 

 consisting of a lipoid membrane and a wealdy staining globule upon it 

 [seeming from the illustrations to be the endosarc spherules], and also 

 a line of granules down the axis of each cilium, figured and interpreted 

 as mitochondria. 



Larson, 1928, reported rearing Opalina in Cleveland's, Putter's, ^ 

 Locke's, and Kinger's solutions, adding egg albumin or blood serum I 

 [not predigested], Putter's fluid plus blood serum seeming the best 

 [worth retesting to see if opalinids do use undigested food]. Adding 

 a bit of rectal wall and subculturing every day or every second day 

 make it possible to maintain a culture a month or more. 



Metcalf, 1928a, discussed with the aid of their Protoopalina para- 

 sites the origin and spread of the bell toads, Discoglossidae. P. 

 stejnegeri, new species, from Ascaphus truei is described. 



Harrison, 1928, discussed host-parasite relations including those of 

 the Opalinidae and their anuran hosts. Reference was made to 



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