MM. Kowalevsky and Barrois on Anchinia. 9 
situated directly upon the wall of the pharyngeal sac, but upon 
a special fold. 
After forming this fold, the layer of flat cells (end) bends 
regularly to the right and left to form the walls of the pharyn- 
geal sac. 
i. Heart and peduncle.—The heart, as usual, is situated at 
the posterior extremity of the endostyle ; it is placed not far 
from the genital organs, and, like the latter, occupies a place 
not distant from the peduncle. 
The stout peduncle which unites the Anchinia to the stolon 
persists during the whole life; it is stopped at its lower part 
by an epithelium thicker than on the rest of the body; and it 
occupies nearly the same position as the stolon of the colonies 
of Pyrosoma. 
II. Gemmation. 
1. The youngest stage observed by us (figs. 2,3) was 
already completely separated from the stolon (fig. 1. no. 3), 
but still possessed a rounded form without any trace of the 
division into body and peduncle. 
When detached from the stolon and examined under a 
greater magnifying-power, this bud presented most of the 
organs already formed—in the middle the pharyngeal sac 
with the endostyle, the cesophagus, and the stomach. Around 
the latter there is a mass of large cells, among which may be 
observed some still more voluminous; between this last and 
the endostyle the pericardium ; and lastly, above the pharyn- 
geal sac and opposite to the endostyle, the ganglionic mass, 
hollowed out by a cavity in its posterior portion. 
It is to be remarked that, in this first stage, the endostyle 
occupies nearly the anterior extremity, the pericardium and 
the ganglionic mass occupy nearly the middle of the two sur- 
faces of the bud; and these relations, which completely alter 
afterwards, are none the less remarkable and worthy of notice. 
Further, we must note the enormous development and the 
peculiar almost spherical form of the endostyle. 
In the early development of the endostyle and its situation 
at the upper part of the bud there is nothing that need greatly 
astonish us; we here find the same thing as in the Salpe. 
Lastly, we have found nothing at this period that can be 
referred to a cloacal sac; moreover, as yet there exists no 
aperture by which the bud is placed in communication with 
the outer world. 
2. In the following stage (fig. 4) the bud has lost its 
rounded form to acquire a clavate form, in which we already 
begin to be able to distinguish a division into body and 
