MM. Kowalevsky and Barrois on Anchinia. 15 
The peduncle of the buds of Anchinia (fig. 7, ¢ p) repre- 
sents a simple elevation of the ectoderm of the bud; it is 
quite circumscribed ; its tissues, which consist of a cylindrical 
epithelium, all belong to the embryo and have no genetic rela- 
tion to the stolon ; at the point of adhesion to the stolon its 
cells are merely superposed upon the cells of the corresponding 
part of the stolon without there being any amalgamation or 
intimate union ; further, the internal cavity of the peduncle is 
completely closed and does not communicate with that of the 
stolon. ‘The buds and the stolon appear to be two distinct 
structures, which are only approximate and in apposition to 
one another, without any direct passage between their tissues, 
and without any amalgamation of the histological elements, 
We might compare these relations to those which exist, in the 
Mammalia, between the uterus and the embryo contained in it. 
The ovum and the embryo to which it gives origin are forma- 
tions independent of the uterus. The ovum is formed in the 
ovary, passess into the uterus, becomes attached thereto for a 
definite purpose ; and there are no genetic relations between 
thetwo. ‘The same thing appears to take place in Anchinia: 
the buds and the stolon have no genetic relations; the buds 
are placed upon the stolon for the purpose of their ulterior 
development; but nothing authorizes us to think that they are 
produced by the stolon ; all our endeavours to detect any rela- 
tion between the buds and the stolon have been in vain ; the 
youngest buds, like the older ones, were always perfectly 
independent of the wall of the stolon, whereas, on the con- 
trary, nothing is easier to prove than the direct relations which 
exist between the organs of the buds of Pyrosoma, the Salpe, 
and the Ascidia, and the corresponding organs of the parent. 
We thus come to the conclusion that the buds of the Anchi- 
nic, in their genetic relations, are independent of the stolon. 
It would be very difficult to explain the apposition of the 
buds of Anchinia if an ingenious discovery of our colleague, 
M. Oulianine, had not given us the key. Formerly M. 
Oulianine discovered that the buds of Doliolum are not formed 
upon the stolon of Doliolum, but that they originate, as in all 
the other Tunicata, at the end of the endostyle, upon the organ 
which has been called the rosette-shaped organ (rosettenfér- 
mige Organ of the Germans). ‘This organ is the true prolife- 
rous stolon of Doliolum; it produces buds in accordance with 
the ordinary process in the Tunicata; these buds separate 
from the rosette-shaped organ, pass to the posterior stolon of 
the Doliolum, become attached to it, and begin to be developed. 
Thus they are genetically quite independent of the stolon 
