16 MM. Kowalevsky and Barrois on Anchinia. 
which bears them. It is to be supposed that an analogous 
process takes place with the buds of Anchinia, and that, after 
being detached from their place of origin, they become im- 
planted upon the stolon, with which we have been unable to 
find that they have any genetic relations. ‘This constitutes a 
further important fact which approximates Anchinia to Dolio- 
lum and separates it from the other Tunicata. 
We may now pass to another point, namely the investi- 
gation of the arrangement of the buds upon the stolon. As 
we have already said, we have met with Anchinia only on 
three different occasions. 
The first time there were brought to us only a few muti- 
lated individuals torn from the stolon: all these individuals 
were well developed; all had perfectly formed ovaries and 
ramified testes filled with spermatozoids, and with the central 
part of an opaque white tint, indicating a state of complete 
maturity. 
The second specimen consisted of a chain about 10 centim. 
long, and bearing buds at different stages of development. 
We omitted to count the number of buds of different ages ; 
but there were rather more young buds than adults (designa- 
ting under the latter name the individuals possessing a com- 
plete intestine filled with food) ; we found no regularity in the 
arrangement of the buds, which, on the contrary, seemed to 
us to be quite irregular: all along the stolon the adult Anchz- 
nie were intermixed with young buds of different degrees of 
development; so that at each pomt one found mixed together 
adult individuals capable of feeding themselves, and young 
buds, the nutrition and growth of which could only be effected 
at the expense of the nutritive substance accumulated in their 
cells (as in embryonic cells in general) or by endosmose, at 
the cost of the nutritive substance contained in the cavity of 
the stolon. 
The presence of adult individuals among the young buds 
reminds one, to a certain extent, of the arrangement of the 
buds of Doliolum. It is well known that there exist on the 
dorsal stolon of Doliolum two kinds of buds—median buds, 
which will give origin to sexuai animals, and lateral buds 
of very peculiar organization ; they possess neither genital 
organs nor proliferous stolon, but have a well-developed 
intestine and branchie which function very early. Gegen- 
baur, Fol, and Grobben have expressed the opinion that 
these lateral buds are more properly respiratory and nutritive 
buds; and it is a fact that their organization is wonderfully 
well adapted to this purpose. Grobben has discussed the 
