18 MM. Kowalevsky and Barrois on Anchinia. 
The first time all the individuals collected had the testes 
much ramified, opaque, swelled with semen; they had been 
captured in the muslin net in stormy weather, and were un- 
fortunately few in number, and all more or less mutilated. 
Our second capture consisted of a well-preserved colony, all 
the individuals of which were mature and presented volumi- 
nous ova; but in them the testes were less developed than in 
the specimens first brought in. 
These observations lead to the supposition that the indivi- 
duals of a colony of Anchinda function sometimes all as males 
and sometimes all as females; it would then be here as in the 
Salpe, among which the chains function successively at first 
as male and then as female chains, owing to the fact that in 
the same chain the male and female organs do not reach 
maturity simultaneously. 
Sexual reproduction is therefore, above all, the purpose of 
the buds of Anchinta. . What is the form to which the ova 
will give origin? This, for the moment, is impossible to 
decide. It will be for ulterior researches to answer this grave 
and important question, the solution of which is no doubt re- 
served for the zoological station of Villafranca, the only spot 
where Anchinia rubra has been observed. 
The individuals which have attained sexual maturity are 
very easily detached from the stolon, as may be often enough 
observed in specimens kept in captivity. Is this the case 
also in a state of freedom? ‘This is difficult to say with any 
certainty, although the analogy with Doliolum renders it pro- 
bable. In any case, whether the adult Anchinie become im- 
mediately detached or remain attached for a certain time to 
the stolon, it is certain that their existence cannot be of long 
duration. ‘The two surfaces in contact, the extremity of the 
peduncle and the corresponding projection belonging to the 
stolon, are only in apposition without intimate union 3 and their 
adhesion is too feeble to resist the movement of the waves 
very long. Now, as the Anchinie do not present any organs 
adapted for pelagic life, they must assuredly perish soon when 
they are detached from the stolon which sustains them. The 
life of the Anchinie must therefore be very short; they perish, 
no doubt, after the deposition of the ova, as may be observed 
in the case of the Salpee. 
The buds of Anchinia must be regarded, like those of the 
Salpe and Doliolum, as representatives of the sexual genera- 
tion, and there must be an agamic generation which produces 
the stolon and gives origin to the buds. 
All the facts revealed in the course of this investigation 
lead us to regard Anchinia as presenting affinities especially 
