PEROPHORA ANNECTENS. 77 



variety. One of these is shown in fig. 36^7. No metotic 

 figures have been seen. 



The variety represented by figure 37 is less reguhir 

 in form, is usually considerably smaller than the pre- 

 ceding, and the cytoplasm is generally almost wholly un- 

 stained, thus rendering the outlines of the cell very in- 

 distinct. The nucleus is relatively considerably smaller 

 than that of the preceding variet}-, containing less nuclear 

 sap. These are the cells that are like the ones found in 

 the test, hence their relations to these latter have already 

 been sufficiently dwelt upon. The next kind of cells — 

 or rather of bodies, for they can hardly be called cells — 

 is represented by fig. 38. They are distinctly marked 

 generally by their large size, their being densely stained, 

 and usually, though not in all individuals, by their irreg- 

 ular form giving them the appearance of having been 

 greatly contracted. And this, I think, is undoubtedly 

 the case since in a few specimens I have found them 

 usually large and regular in outline. In the shrunken, 

 deeply stained ones, no nuclei can be seen; but in the 

 ones not thus shrunken and stained a relatively small, 

 well stained nucleus is present. The plump ones, as 

 they ma}^ be called, are very large — several times the 

 volume of the largest ones of the first described variety — 

 and the protoplasm has a fine granular structure. 



The fourth variety, shown in figures 35, 35^, 35*^ and 

 35c seem to correspond to what are usually described in 

 other Tunicates as pigment cells; though if the granules 

 which are so conspicuous in them are pigment, which 

 I greatly doubt, they are certainly, in this species, quite 

 different from anv pigment known to me ; they seem to 

 me to be fragments of the broken-down cell body. In 

 h^emvtoxalin they become intensely stained, but some 

 other stains, e. g., Mayer's cochineal, attack them very 

 little or not at all. I am pretty well convinced that these 



