1924] 
WOLPERT—FUNGI AND H-ION CONCENTRATION OF MEDIA 51 
2? C. in a mixture of 520 cc. of КХО; of 1.1 specific gravity and 
30 gms. of KClO, (Zeller, Schmitz and Duggar, '19). After 
washing and precipitating in Schweitzer's reagent, according to 
McBeth (716), an abundance of flocculent cellulose was obtained. 
This was washed repeatedly in distilled water until free from 
copper. The washing process was hastened by centrifuging and 
decanting, the necessarily long periods of time required in the 
usual settling method being avoided. 
TABLE III 
THE amd sod OF THE MODIFIED RICHARDS' E SOLUTION CONTAINING 
LLULOSE ee TO A RANGE ee Рн FROM 3.0 TO 
0 AT INTERVALS OF 
Tatal М№о. се. | No. се. No. сс. | №, сс. | No. се. | No. се. 
Р M/8 M/3 м/д nutrient | cellulose total 
HPO; KH;PO,| K;HPO, | solution | solution | solution 
3.0 1.2 5.8 14 14 35 
4.0 0.1 6.9 14 14 35 
5.0 6.8 0.2 14 14 35 
6.0 5.0 2.0 14 14 35 
The Richards’ solution with cellulose was adjusted to the 
desired Рн according to table ш. As in the previous cases this 
table required some slight adjustment for each series. Тһе 
phosphates constitute 20 per cent of the total volume; the cel- 
lulose solutions, 40 per cent; and the nutrient salts, 40 per cent. 
Therefore all salts were weighed on the basis of 100 per cent, or 
1000 се. of solution, and dissolved in enough doubly distilled 
water to give a total of 400 cc.—40 per cent of the total amount. 
Fourteen-cc. amounts of this solution were added to each flask. 
The proper balance was obtained by the addition of 7 cc. of 
phosphate solutions and 14 cc. of the cellulose suspension to each 
flask containing this concentrated nutrient salt solution. The 
КН.РО, was the only phosphate added before sterilization. 
The peptone-filter-paper solution was adjusted to the desired 
initial Py according to table п. The reduction in the amount 
of peptone did not change the amount of phosphates required 
to obtain the initial Pg. The strips of filter-paper were added in 
equal amounts to each flask. On the basis of 20 gms. of paper 
per liter of solution, each culture flask received approximately 
0.7 gm. of paper. 
