1886.] 



MICROSCOPICAL JOURNAL. 



33 



173. V. Thanhoffer. Das Mikroskop 



und seine Anwendung. 

 Stuttg., 1880. 



The tissue is taken from the silver 

 solution and put in a dry dish, while 

 a 2% solution of acetic acid is 

 dropped on it continuously with a 

 brush while exposed to the light. 



His pupil, Krauss, has devised a 

 peculiar method. The material is 

 taken from the silver, washed and 

 put in a bright red solution of potas- 

 sium permanganate. The reduction 

 is rapid, even in the dark. Some- 

 times failures occur. It is even pos- 

 sible to mix the fluids. Another 

 student, Carl Oppitz, uses silver 

 nitrate and stannic chloride. Prep- 

 arations treated as usual with silver 

 are laid for two or three minutes in 

 a 4-^% solution of stannic chloride, 

 in which they are carefully agitated. 

 Reduction is rapid and the precipi- 

 tate very fine grained. Impregnation 

 with chloride of gold or chloride of 

 gold and potassium. 



174. Cohuheim. Ueber die Endi- 



gungen der sensiblen iierven 

 in den Hornhaut. Arch, pa- 

 thol. Anat. w. Physiol., 

 xxxviii, 343. 

 Chloride of gold is substituted for 

 silver nitrate in a similar process. 

 The metal is rapidly reduced by the 

 action of light on organic tissues. 

 These become yellow, then red, and 

 finally a bluish black. A |-% solu- 

 tion of gold chloride is applied, and 

 then several days of soaking in water 

 acidulated by acetic acid. Mount in 

 glycerin or balsam. Different cells 

 vary in intensity and color. Glands 

 redden quickly. Many nuclei re- 

 main colorless. Nerve elements of 

 both kinds color more quickly than 

 protoplasm. Capillaries become red, 

 but epithelium and cement substance 

 do not take the color. 



175. Arnold. Ein Beitragzu derfein- 



eren Structur der Ganglien- 

 zellen. Arch. path. Anat. u. 

 Phys., xli, 17S. 

 The chloride of gold and potas- 

 sium is dissolved in a 1% sol. acetic 



acid and the preparation treated w^ith 

 a bath of this mixture of 02.0-0.05 

 % strength. In 3-4 hours, or as 

 soon as a violet tint appears, change 

 to a I/O acetic acid in which the ma- 

 terial may rest for 3-5 days till it 

 assumes a deep color. This method 

 is particLilarl}^ adapted to show the 

 nerve filaments in the ganglions ; 

 after the connective tissue is dissolved, 

 moisten with glycerin, to w^iich a 

 little acetic acid is added, and expose 

 to the light on a slide resting on a 

 white surface. In 4 to 5 days the 

 ganglion cells will be intense, the 

 muclei clear, the nucleoli feebly red, 

 the axis and thicker nerve fibres 

 bright red, but after 8-10 days even 

 the finer fibres take an intense color. 



176. Cui'voisier. Ueber die spinulen 



und sympathischen Zellen des 



Frosches. Centralbl. f. d. 



med. Wiss., 1867, No. 57. 



A simpler method than the last. 



A sympathetic ganglion is slightly 



crushed or pulled apart, then laid for 



^— I day in 0.2% acetic acid, dissected 



on a slide, and treated continuously 



with a few^ drops of gold chloride, 



o.i/o sol. while exposed to the light. 



(This process succeeds much better 



in a moist chamber) . 



177. Bastian. 



Dissolves I pt. gold chloride in 

 2000 dist. water, adds a drop of hy- 

 drochloric acid. Reduction takes 

 place in a mixture of equal parts 

 alcohol and formic acid. The opera- 

 tion may be hastened by heat, and 

 our author has also made double 

 stainings of silver and gold. 



178. Nathusius. Ueber die Mark 



substanz verschiedener Horn- 

 gebilde, etc. Arch. f. Anat. 

 Jahrg., 1869, p. 69. 

 Chloride of gold is used in a solu- 

 tion of 0.005 to i*^^ ^^ water. The 

 sections are reduced by a solution of 

 subsulphate of iron. 



179. Gerlach. Artikel Riickenmark 



in Strieker's Handbuch der 

 Gewebelehre, 1871, p- 678. 

 In the examination of the spinal 

 marrow^ potassium gold chloride is 



