34 



THE AMERICAN MONTHLY 



[February, 



preferred. The organ is hardened in 

 ammonium bichromate, then put in 

 solution of gold chloride (i-ioooo) 

 to which a little hydrochloric acid is 

 added. It takes 10-12 hours to bring 

 out a light lilac hue, then wash in 

 acidulated water, and finally in 60% 

 alcohol, likewise slightly acidulated. 

 (Gerlach's gold-stained preparations 

 are, with respect to the finer nerves, 

 unsurpassed and rarely equalled. 

 The acid fuchsin process of Weigert 

 alone can compare with it) . 



180. Henoque. Du mode de distribu- 



tion et de la terminaison des 

 nerfs dans les muscles lisses. 

 Arch, de I'Anat. et Physiol., 

 1870. 



181. Klein. Beitrag zvu' Kenntniss 



der peripherischen Verzwei- 

 gung markloser Nerventasern. 

 Centralbl. f. d. med. Wiss., 

 1871, No. 38. 

 Derselbe. On the peripheral distri- 

 b u t i o n of non-medullated 

 nei^ve fibres. Qriart. Journ. 

 Microsc. Sci., vol. xi, p. 40^ : 

 vol. xii, p. 30I . 

 183. Chrchtschonovitsch. Beitrage 

 zur Kenntniss der feineren 

 Nerven der vaginal schleim- 

 haut. Weiner Acad. Sitzber, 

 1871, Abth. ii, Februar, p. 

 301. 

 All three recommend for verv fine 

 nerves and their branches a particular 

 gold method. Portions of the fresh 

 organ are placed for 30-45 minutes 

 in a -|-/o solution of gold chloride, 

 then for 13 to 24 hours in distilled 

 water. They are then treated with 

 an almost saturated solution of tar- 

 taric acid in a well-corked flask. 

 Klein and his pupil, Chrchtschono- 

 vitsch, set the vessel in warm water 

 of 50° C. and allow all to cool. 

 Henoque heats the water to boiling, 

 which is thought to injure the epi- 

 thelimn by K. and Ch. The brown 

 or violet pieces of tissue are cut in 

 fine sections in which the nei^ve ram- 

 ifications may be clearly seen. 

 183. Boll. Die Histologic und His- 

 tiogenese der nervosen Cen- 



tralorgane. Arch. f. Psych, u 



Nervenkr., iv, 52. 

 Contains more precise directions on 

 Gerlach's gold and potassium chloride 

 method. The staining is better, the 

 shorter the time of exposure to the 

 ammonium bichromate. The ma- 

 terials do not stain well 8 days old, 

 after 14 days they are worthless. Al- 

 cohol should not be used even to 

 moisten the razor lest it cause a pre- 

 cipitate. The quantity of solution 

 (i-ioooo) need not be so large, and 

 the sections should not lie in it over 

 18 hours; 12 is usually the best time. 



184. Lawdowsky. Bemerkvmgen 



zur mikroskopischen Technik. 



Med. Bote, 1874. No. 37-39; 



Russisch. 

 Expresses dissatisfaction with or- 

 dinary gold stainings, and recom- 

 mends a modification introduced by 

 Nesteroftski in Kieft', which consists 

 in reducing by ammonium sulphide. 

 Each section requires about a drop, 

 which is soon removed by blotting- 

 paper and glycerin substituted. The 

 preparations are very clear and trans- 

 parent, the metallic precipitate being 

 dissolved. They should be kept in 

 the dark. The method is especially 

 adapted to sho^v the network of 

 nerves in the walls of the colon, the 

 nerve endings in the muscles, and the 

 large central nerves. 



185. Lowit. Die Nerven der glatten 



Musculatur. Wiener Sitzber, 

 Ixxi, April, 1875. 



186. Fischer. Ueber die Endigungen 



der Nerven im quergestreiften 

 Musk el der Wirbel thiere. 

 Arch, mikrok. Anat., xiii,356. 

 To show nerve terminations in 

 muscles, make a 1% solution of gold 

 chloride, and a mixture of i pt. for- 

 mic acid and 3 pts. dist. water. A 

 few c.c. of the last are put in a watch- 

 glass, and pieces of the tissue under 

 examination i to 3 mm. thick are 

 dipped in for ^ a minute till trans- 

 parent. They are then dipped for 

 10-15 minutes in gold chloride till 

 they are quite yellow. Then in 

 dilute formic acid in the dark, (i pt. 



