1886.] 



MICROSCOPICAL JOURNAL. 



35 



acid, 2 of water) , and then for 24 

 hours in pure formic acid in the 

 dark. Finally wash well in distilled 

 water and mount in glycerin. 



187. Thin. A contribution to the 



anatomy of the lens Journ. 



Anat. and Phys., x, 229. 

 A solution of gold chloride ^% is 

 forced into the aileries until the tis- 

 sues are saturated. The pieces are 

 then laid in a similar solution for a 

 short time and may then be tinted 

 with hematoxylin. 



1 88. Flechsig. Die Leitungsbahnen 



im Gehirn und Riickenmark 

 des Menschen. Lpz., 1876. 

 The large nerves are first put in a 

 1% solution of ammonium bichro- 

 mate. When hard enough to cut 

 well into sections, wash, and put into 

 0.5% gold chloride for :^ to 4 hour, 

 wash well and transfer to 10% solu- 

 tion of caustic soda. The reduction 

 is almost instantaneous, the white 

 substance becomes dark violet, the 

 gray remains colorless. After laying 

 for some hours in the soda, the prep- 

 arations are thoroughly washed, 

 and mounted in Canada balsam as 

 usual. 



189. Ranvier. Legons sur I'histolo- 



gie du svsteme nerveux. Paris, 

 1878. 

 To bring ovit the nerves of the 

 cornea, lay it for five minutes in fresh 

 filtered lemon juice, then for 15-20 

 minutes in 3 c.c. of a 1% solution 

 gold chloride, and finalh^ in distilled 

 water to which a drop of acetic acid 

 has been added. Reduction follows 

 after exp<^sure to the light for 2-3 

 days, and the fabrillce of the nerves 

 show clearly. To bring out the nerve 

 terminations in the muscles, the 

 method is to be modified by trans- 

 ferring the sections of muscle from 

 the solution of gold chloride to a 

 3o"o solution of formic acid for 12 

 hours in the dark. 



190. Hoggan. Combination of sil- 



ver nitrate and gold chloride. 

 See No. 168. 



[_To be conti>med.] 



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Dr. Carpenter's Portrait. — 

 We regret to announce that the plate 

 engraved for us, proofs of which had 

 been received and approved, was 

 destroyed by the recent large fire in 

 Philadelphia. The plate was in the 

 possession of Messrs. Crosscup & 

 West, who had prepared it with 

 especial care and correspondinglv 

 excellent results ; but their entire 

 establishment was consumed on the 

 night of the 25th, if we recollect 

 aright, with a loss to the firm of be- 

 tween $15,000 and $20,000. 



Under the circumstances we trust 

 our readers will accept this unavoid- 

 able delay in good part, and as for 

 ourselves we are not disposed to urge 

 the production of a new plate until 

 the firm have had an opportunity 

 to recover somewhat from the inevi- 

 table consequences of such a misfor- 

 tune. We hope to have another 

 plate in time for the April issue, if 

 not before. 



The Limits of Resolution. — 

 In the last number of the Journal of 

 the Royal Microscopical Society, 

 Mr. Frank Crisp has treated this 

 subject in his usual able and lucid 

 manner. The results are of consid- 

 erable interest. 



Referring to the formula which 

 gives the limit of resolution for any 



