1886.] 



MICEOSCOPICAL JOURNAL. 



53 



called agamospores. In other gen- 

 era, spores may be formed without 

 copulation, but such spores are pro- 

 duced in cells capable of conjugating, 

 and since in these instances the cells 

 may be regarded as possessing cer- 

 tain sexual characters, the spores 

 thus formed are knoy^n as partheno- 

 spores.] 



88. Genus S t a u r o s p e r in u m 

 Kiitzing. 



Copulation geniculate ; sporocar- 

 pium formed by quinqui partition 

 of the H-shaped zygospore, short- 

 cylindric, quadrangular in one view% 

 elliptic in the other. Pericarpium 

 four-celled. 



89. Genus Plagiospermum Cleve. 

 Copulation by one cell bending in 



a geniculate manner and the other 

 sending out a lateral tube to the 

 angle, where the spore is formed. 

 Sporocarpium formed by quadri- 

 partition of the zygospore. Peri- 

 carpium three-celled. 



90. Genus JSIesocarpjis Hassal. 

 Copulation ladder-like, sometimes 



lateral. Carpospore spherical or 

 oval, between the straight or some- 

 what cui"ved copulating cells. 

 Pericarpium two-celled. 



91. Genus Craterospermum A. 

 Braun. 



Copulation geniculate ; spore with 

 a spherical interior, and short-cylin- 

 dric, nearly square exterior with an 

 annular furrow and concave ends. 

 Pericarpium two-celled. 



[This genus is combined with the 

 sub-genus A/esoca?pi/s by Wittrock.] 

 [To be continued.'^ 

 O 



Staining Tissues in Microscopy. — 

 IX. 



BY PROF. HANS GIERKE. 

 \^Co7i tinned from p. JJ.] 

 191. Carriere. Kurze Mittheilun- 

 gen zur Kenntniss der Herbst- 

 'schen und Grandry'schen 

 Korperchen in dem Schnabel 

 der Ente. Arch. mikr. Anat. , 

 xxi, 146-164. 

 Carriere employs a method sug- 

 gested by von Bohm, as follows : — 



The pieces are laid in 50 per cent, 

 formic acid till, in about 20 minutes, 

 they become transparent, then 

 washed, and put for about 20 min- 

 utes in a small quantity of gold chlor- 

 ide I per cent, solution. They are 

 again v\^ashed and laid for 24 hours 

 in the dark in Prichard's mixture of 

 amyl alcohol and formic acid each 

 one part to 98 of water. 



192. Marchi. Ueber die Terminal- 



organe der Nerven in den 



Sehnen der Augenmuskeln. 



Arch. f. Opthalm., 28, Jahrg. 



i, 202-21 ; auch Arch, per le 



Scienze med.. v. 

 Marchi recommends Manfredi's 

 process, which consists in soaking 

 the fresh tissue for half an hour in a 

 one per cent solution of gold chlor- 

 ide, then in 0.5 per cent, solution of 

 oxalic acid at 36° C. temperature, 

 in which the tissue remains till cold. 

 Another method, suggested by 

 Golgi, for the examination of nerve 

 endings in the muscles, consists in 

 treating them for three days in a 2 per 

 cent, solution of potassimn bichro- 

 mate, then for 30 minutes in a i per 

 cent, solution of arsenious or acetic 

 acid, and for the same time with a i 

 per cent, gold chloride liquor, and 

 after washing again with arsenious 

 acid in which the preparation is ex- 

 posed to the light. 



193. Bremer. Ueber die Endigun- 



gen der markhaltigen und 



marklosen Nerven im querge- 



streiften Muskel. Arch. f. 



mikr. Anat., xxi, 195. 



A slight modification of Lowey's 



method. Lay first in 25 per cent. 



formic acid till transparent, then 15- 



20 minutes in a i per cent, gold 



chloride solution, then again in the 



formic acid in the dark. The latter 



is changed for a solution of formic 



acid of double strength for 24 hours, 



and finally for 2-3 weeks in 20 per 



cent, formic acid glycerin till the 



proper degree of consistency and 



depth of color is reached. 



Treatment with perosmic acid. 



