1886.]- 



MICROSCOPICAL JOURNAL. 



71 



till sufficiently black. Applied to 

 ganglia, the nerve elements are 

 stained while connective tissue re- 

 mains colorless. 

 203. Fr. Eilh. Schulze. Eine neue 



Methode der Erhiirtung vuid 



Farbung thierischer Gewebe. 



Centralbl. f. d. med. Wiss., 



1867, No. 13. 

 Palladium chloride is particularh' 

 recommended for hardening and 

 staining, especially for muscular tis- 

 sue, also for the cells of glands and of 

 epithelium containing granular pro- 

 toplasm, while all connective tissues, 

 fat, etc., remain colorless. Pieces of 

 material as large as a bean are put in 

 a solution of 1-800 to 1-1500 ; about 

 i-iooo is best. In 34 hovu-s they 

 may be cut into sections, and will be 

 found a golden yellow. They may 

 afterward be stained red with ammo- 

 niacal carmine. 



304. Bastian. Recommends palla- 



dium highly when used ac- 

 cording to 117, 1S69. 



305. Leber. Zur Kemstniss der Im- 



pragnations -met h o d en d e r 

 Horn h a u t u n d ;i h n 1 i c h e r 

 Gewebe. Arch. f. Opthalm. 

 xiv, 300. 

 In examinations of the cornea, 

 various metallic salts besides silver 

 were employed. A combination of 

 potassium ferridcyanide and ferrous 

 salts was deemed preferable. The 

 fresh cornea of a frog is laid for five 

 minutes in a | to 1% solution of a 

 ferrous salt, carefully deprived of 

 epithelium, thoroughly washed and 

 transferred to a 1% solution of potas- 

 sium ferridcyanide in which it is 

 shaken till deeply blue. The same 

 result may be attained by precipita- 

 tion from a 2% solution of ammonio- 

 cupric sulphate with slight excess of 

 ammonia and a ^% solution of potas- 

 sium ferrocyanide. Plumbic acetate 

 and potassium chromate give a yel- 

 low stain. 



306. Henle und Merkel. In Henle's 



Handbuch des Nervenlehre 

 des Menschen. 1871. 

 Sections of large nerves are laid in 



solutions of palladium chloride, 1-300 

 to 1-600, till they acquire a strav^ 

 yellow color, which takes 1-3 min- 

 utes. They are then placed in am- 

 moniacal carmine. 



307. V Thanhofler. D a s Mikroskop 



und seine Anwendung. 1880, 



P- 143- 

 Recommends palladium chloi'ide 



to stain the nerves of the cornea. 



308. Golgi. Un nuovo processo di 



tecnica microscopica. Rendic. 



R. instituto Lombardo, xii, 



306-310. 

 Pieces of the larger nerves 1-3 

 cm. in diameter are hardened in 

 MoUer's fluid or in potassium bichro- 

 mate. After 15-30 days they are 

 put in 0.35 to 0.5% solution corrosive 

 sublimate. This must be renewed 

 daily for S-io da^ys, when the reac- 

 tion is complete. The pieces are 

 colorless and have the appearance of 

 fresh horn. The finished sections 

 are well washed a n d mounted in 

 glycerin or balsam. The reaction 

 affects the ganglion cells and their 

 processes, also unstriped muscle. 

 The elements appear white by 

 reflected, and black by transmitted, 

 light. The best results were ob- 

 tained from the cortex of the cere- 

 brum, less satisfactory from that of 

 the cerebellum, and none from the 

 spinal marrow. 



COMBINATION METHODS. 



309. M. Schulze und Rudnefl". See 



194. 

 Preparations of O s m i u m are 

 stained in ammonia carmine. 



3 10. Fr. Eilh. Schulze. See 303- 

 Preparations of palladium chloride 



are tinged with carmine. 



311. Henle und Merkel. See 206. 

 Nerve sections treated as per 210 



in strong solution of ammonia- car- 

 mine become bright red in the cen- 

 tral axis while the gray matter re- 

 mains yellow. 



213. Schwarz. Ueber eine Methode 

 doppelte Farbung mikroscop- 

 ischer Objecte und ihre An- 

 wendung, etc. Sitzber, d. k. 

 Acad. d. Wiss. Wein, Iv. 



