72 



THE AMERICAN MONTHLY 



[April, 



The preparations are treated* first 

 v^ith carmine then with picric acid. 

 The material is then placed in a 

 mixture of i part creosote, lo pts. 

 vinegar, and 20 pts. water, boiled 

 one minute, dried and cut into sec- 

 tions, which are put for an hour in 

 dilute vinegar, then washed and 

 stained with a rose color solution of 

 carmine, washed again and laid for 

 two hours in a solution of picric acid 

 0.066 gm. to 400 c.c. water. Mount 

 in dammar. Muscles, cell contents, 

 vessels, and nerves will be yellow, 

 connective tissue and nuclei red. 



213. Ranvier. Technique mikro- 



scopique. Arch, de Phys., 

 186S, No. 2, p. 319; No. 5, 

 p. 666. 

 Highly recommends a mixture of 

 picric acid and ammoniacal carmine. 

 The fluid should look like gooseberry- 

 juice and, as it is liable to mold, 

 should be kept corked with a cork 

 soaked in camphor tincture. 



214. Strelzofl'. Zur Lehre der 



Knochenentwickelung. Cen- 



tralbl. f. d. med. Wiss., 1S73, 



No. iS, p. 277-78. 

 Derselbe. Ueber die Histogenese 



des Knochens. Unters a. d. 



pathol. Inst., Zurich, 1S73, 



p. 1-94. 

 In the studv of the development ot 

 bone, neutral carmine and htema- 

 toxylin are found to dye calcified 

 substance blue, new formed material 

 red. (Unfortunately these beautiful 

 preparations are not permanent, the 

 hcEmatoxylin bleaching in a few 

 3'ears.) 



215. Rouget. Cfr., 162. 

 Preparation treated with silver 



are placed for 2-3 hours in a mixture 

 of ammonia carmine, glycerin, and 

 alcohol. 



216. Merkel. Technische Notiz. 



Unters. a. d. Anat. Anst. 



Rostock, 1874, p. 98. 

 Dyes the brain and spinal marrow 

 in a mixture of carmine and indigo 

 carmine. The gray matter becomes 

 blue, blood corpuscles green, the 

 rest red. Bones decalcified in Mol- 



ler's 'fluid and hydrochloric acid be- 

 come blue in the formed material, 

 the rest red. 

 217. Baber. E. Cresswell. Note on 



picrocarminate of ammonia. 



Q^uart. Journ. micr. sci., 



1874, p. 251-3. 

 Repeats the statements of Schwarz 

 and Ranvier. 

 21S. Duval. Proc^de de coloration 



des coupes du systeme nerveux, 



Journ. de I'iVnat, 1876, p. Ill 



—112. 

 Applies carmine the usual way, 

 transfers to alcohol, then for 10-12 

 minutes in anilin blue (10 drops sat. 

 sol. to 10 grains absolute alcohol) 

 and mounts in balsam. Nerve cells 

 and nerve axis stain reddish violet, 

 the vessels violet blue. Connective 

 tissue, the pia mater and its pro- 

 jections become blue. 



219. Norris and Shakespeare. A 



new method of double stain- 

 ing. Amer. Journ. Med. 

 Sci., 1877, January, and, — 



220. Merbel. Double staining with 



a single fluid. Monthly Micr. 



Journ., 1S77, Nov. and Dec, 



p. 242. 

 Two solutions are made. A, car- 

 mine 2, borax 8, distilled water 130 

 parts. B, indigo carmine 8, borax 

 8, distilled water 130. Rub in a 

 mortar, filter and mix equal parts of 

 the two solutions. Lay sections a 

 few minutes in alcohol, then 15—20 

 minutes in this mixture, and an equal 

 time in a saturated solution oxalic 

 acid, wash and mount in balsam. 

 The fundamental part of connective 

 tissue, cartilage and bone will be 

 blue, cell structure red, ganglion cells 

 purple, tbeir nuclei red, and the nu- 

 cleoli blue, the sheath of the nerve 

 axis blue or green, the axis cylinder 

 green. 



221. vSchieflerdecker, Kleinere his- 



tologische Mittheilungen, II 

 Ueber eine neue Farbungs 

 methode des Central nerveu- 

 sy stem s . Arch . M i k r o s k . 

 Anat. XV, 38. 

 Henle and Merkel published in the 



