1886.] 



MICROSCOPICAL JOUENAL. 



73 



Handbuch der Anatomie the first 

 method of double staining with pal- 

 la d i u m chloride and ammoniacal 

 carmine. Schiefterdecker replaces 

 the last by sodium picrocarminate, 

 in a cold saturated solution of which 

 the sections remain for S-io minutes 

 after 1-3 minutes treatment with pal- 

 ladium chloride. Mount in shellac 

 or balsam. The preparations darken 

 after mounting. The sodium picro- 

 carminate alone is good for staining 

 ganglion cells. 



222. Klemensiewicz. Beitriige zur 

 kenntniss der Farbenwechsels 

 der Cephalopoden. Sitzber. 

 d. Acad. d. Wiss. Wien, xxviii, 

 (1878). 

 Rub together i grn. carmine ^vith 

 30 drops concentrated ammonia and 

 dilute with 200 c.c. water. Mix two 

 parts of this carmine with one part 

 cold saturated solution of picric acid 

 and heat on the water bath S-io hours. 

 Add dilute ammonia to replace loss 

 if necessary and evaporate to ^ 

 or ^ of first quantity. Little or no 

 precipitate should appear on cooling. 

 The clear liquid when finished should 

 be dark red in deep layers with a yel- 

 lowish cast in their strata. 

 333. Lang. Line neue Tinctions 

 methode. Zool. Anz. ii 

 (1879), 45. 

 Double staining by eosin and pi- 

 crocarmine is much praised, since it 

 penetrates whole animals and differ- 

 entiates not merely nuclei and simi- 

 lar bodies but also the protoplasm of 

 ganglia cells and nerve fibres. Mix 

 a i"o solution of picrocarmine and a 

 2% solution of eosin in water. Soak 

 whole specimens (Planaria) in this 

 mixture 4 days, then in 70% alcohol, 

 then in 90^0 till no more color is ex- 

 tracted. 



224. Seller. Practical hints on pre- 

 paring and mounting animal 

 tissues. Am. Mo. Micr. 

 Journ., i, 220. 

 The solutions, (a), carmine i.o 

 grn., borax 3.5 grn., aq. dest. 150 c.c, 

 95% alcohol 330 c.c; (b), hydro- 

 chloric acid 1.0 grn., alcohol 4 c.c. ; 



(c), solution sodium sulphindigotate 

 2 drops, 95"o alcohol 330 c.c. So- 

 dium sulphindigotate is made by di- 

 gesting the best Bengal indigo with 

 fuming sulphuric acid, washing out 

 excess of acid, and precipitating with 

 salt. The well-w^ashed precipitate 

 is dissolved in warm distilled water 

 to saturation. The preparations are 

 cleared up with benzol and finally 

 mounted in alcohol balsam. 

 235. Gage. Preparation of Ranvier's 



picrocarmine. Amer. Monthly 



Micr. Journ., i, 22. 

 Dissolve I pt. carmine in ^o pts. 

 strong ammonia, and i part picric 

 acid in 100 pts. water. Mix both 

 solutions, evaporate at 45° C. to ^ 

 volume, filter through double paper 

 and dry. A solution of powder, i 

 pt. to 100 should be clear. If not 

 after filtering, standing several days 

 and again filtering, add the ammonia 

 in equal quantity again, and evapor- 

 ate. If clear add to each 100 c.c 

 25 c.c. pure glycerin, and 10 c.c. 

 95% alcohol. This solution is very 

 permanent. 

 226. Mayer, P. See No. 28. 



Picrocarmine is recommended as 

 staining more precisely than any 

 other color. It is prepared by adding 

 a concentrated water solution ot 

 picric acid to ammoniacal carmine 

 (2.25) till a precipitate begins to fall. 



337. Neumann. Die Pikrocarminfar- 



bung und ihre Anwendung auf 



die E n t w i c k e 1 u n g s 1 e h r e . 



Arch. Mikr. Anat., xviii, 130- 



161. 

 To avoid the occasional failures of 

 picrocarmine, treat sections stained 

 by Ranviei''s method with acidulated 

 glycerin (i pt. hydrochloric acid to 

 300 glycerin), and control the reac- 

 tion by the aid of the microscope, and 

 then mount in glycerin. 



338. Richardson. Section of larynx 



of h u m a n foetus . Q_u art. 

 Journ. Micr., 1880, p. 113. 

 Describes a combination of car- 

 mine, picric acid, and madder. 

 [Zb be continuedJ^ 



