98 



THE AMEEICAN MONTHLY 



[May, 



the sections are put in acidulated 

 water, (acetic or picric acid), then in 

 hsematoxylin solution. This method 

 is very successful for cell division and 

 the development of epithelium and 

 spermatozoa. 



Carmine indigo-carmine is also 

 recommended. Take of carmine 2 

 pts., borax 8, water 30. Soak in this 

 for a few minutes, then in acid alco- 

 hol (i-3o). If they become a rose 

 red, M^ash in methyl-alcohol, and 

 treat with indigo-carmine till blue. 

 A saturated solution of indigo-car- 

 mine is poured into methyl-alcohol 

 till a deep blue results, and is then 

 filtered. Picrocarmine is also com- 

 bined with other anilin colors, by 

 treating first with picrocarmine, then 

 with the anilin. 



230. Stirling. On double and treble 



staining of microscopic speci- 

 mens. Journ. Anat. a Phys., 

 >^v, 349-354- 

 Picrocarmine is recommended for 

 blood corpuscles and epithelium after 

 treatment with osmic acid. 



Picric acid may be used for harden- 

 ing, and afterwards picrocarmine for 

 staining. This method succeeds well 

 with blood corpuscles, elastic tissue, 

 and cartilage which color vellow, 

 while the connective tissue becomes 

 red. Foetal bones decalcified in pic- 

 ric acid, and bone corpuscles, color 

 red, but the harder parts yellow. In 

 the large arteries, connective tissue 

 becomes red, elastic tissue yellow, 

 unstriped muscle fibres yellow 

 brown. The combination of hasma- 

 toxylin and picrocarmine is recom- 

 mended for skin, unstriped muscles, 

 and the development of bone, and 

 finally picrocarmine gives excellent 

 results in combination with anilins, 

 as iodine green. 



231. Weigert. Zur Technik der 



mikroskopischen Bacterienun- 



tersuchung. Arch. path. Anat. 



a Phys., Ixxxiv, 275-315. 



Four grams ammonia are poured 



on two grams carmine and protected 



from dust 24 hours, then 200 g. cone. 



sol. picric acid is added, and after 24 

 hours more a little acetic acid till a 

 precipitate appears. At intei-vals of 

 24 hours, ammonia is added by drops 

 till the solution is clear. ' If the stain 

 is too red, add a little ammonia, if 

 too yellow, a little acetic acid. 



232. Richardson. Multiple staining 



of animal tissues with picro- 

 carmine, iodine, and inalachite 

 green dyes, and of vegetable 

 tissues with atlas scarlet, solu- 

 ble blue, etc. Journ. R. Micr. 

 Soc, i, 868-872. 

 (See No. 228 for an earlier mix- 

 ture) . Three solutions are described 

 for animal tissues: — (a), picrocar- 

 mine with a thin transparent solution 

 of equal parts of iodine and mala- 

 chite greens; (b), the same with 

 malachite green in excess; (c), pi- 

 crocarmine and malachite green alone. 



233. Hover. The carmine described 



in No. 30 is dissolved in a 

 neutral concentrated solution 

 of ammonium picrate. 



234. Boiuiet. Zur mikroskopischen 



T e c h n i k . Dtsch Zeitsch f. 

 Thiermed, vii, 301—303. 



lI.EM ATOXYI. IN AND METALLIC- 

 SALTS, ETC. 



23 V Gcrlach. Structur der gefliss- 

 biiute vSitzber. d. plays, med. 

 Soc. Erlangen, 1872. 

 Transverse sections of dried ves- 

 sels are laid in a weak solution of 

 hiematoxylin to which a little aluin 

 has been added, and when they are 

 blue enough they are transferred for 

 a few minutes to pure acetic acid, 

 and then for the same time to dilute 

 picric acid. They are then washed 

 and mounted in balsam or glycerin. 

 Unstriped fibres and nuclei become 

 violet, connective tissue reddish 

 brown, and elastic fibres straw yel- 

 low. 



236. Eberth. Experimentelle Unter- 

 suchungen uber der Entziin- 

 dung der Hornhaut. Unters 

 d. pathol. Inst. Zurich, ii, 

 (1874), 1-58. 



