150 



THE AMEEICAN MONTHLY 



[August, 



Staining Tissues in Microscopy.— 

 XI. 



BY PROF. HANS GIERKE. 



{^Continued from page gg.'\ 



239. Busch. Die Doppelfarbung cles 



Ossificationsrandes mit Eosin 



and hsematoxylin. Verhandl. 



d. Berl.'Phys. Ges., 1877, No. 



14. 

 Sections of decalcified bones are 

 placed for some days in a \X chromic 

 acid or 1% potassium bichromate, 

 carefully washed and put into eosin 

 solution. When sufficiently stained 

 they are put in hsematoxylin. Funda- 

 mental cartilage appears light blue on 

 the edges of ossification, the nuclei of 

 the neighboring cartilage cells are 

 red, the contents of the medullary 

 cavity light red, while the formed 

 bone is a mixed tint between blue and 

 red. 



240. Renaut. Sur I'eosine-h^matoxy- 



lique et sur son emploi en 

 histologic. Compt. rend., 

 Ixxxviii, 1039-1042. 

 Equal parts of neutral glycerin and 

 a saturated solution of eosin in alco- 

 hol or water are mixed, to which is 

 added by drops Bohmer's hccmatoxy- 

 lin (see No. 37) till the green fluoV- 

 escence can scarcely be perceived. 

 These stainings are mounted in salted 

 glycerin (i-ioo) or in balsam. If 

 the latter, they should be treated with 

 alcoholic eosin (absolute) or clove 

 oil. Good results are obtained from 

 material hardened in alcohol, chromic 

 or osmic acid. Nuclei stain violet, 

 connective tissue gray, elastic fibers 

 and blood corpuscles dark red, cell 

 protoplasm and nerve axes rose color. 

 The secretory cells of the salivary 

 glands stain blue, their nuclei violet, 

 and the crescent of Gianuzzi deep 

 red. 



241. Brandt. Farbung lebender ein- 



zelliger Organismen Biol. 



Centralbl., 1881, p. 202. 

 In staining unicellular organisms 

 hsematoxylin and Bismarck brown 

 may be combined with success. 



242. Renaut. Sur le mode de prep- 



aration et I'emploi de I'eosine 



et de la glycerine hema- 



toxylique en histologic. Arch. 



le Phys., 1881, p. 640. 



Another method similar to No. 240. 



Make a sattu-ated solution of eosin 



in salted glycerin and mix with a 



glycerin saturated with potash alum. 



Filter and add alcoholic hgematoxylin. 



Mount with treatment as in 240 or in 



the staining fluid itself. 



243. Stirling. See 230. Haematoxy- 



lin and iodine green or eosin. 



ANILIN COLORS WITH METALLIC 

 SALTS. 



244. Lawdowsky. See No. 88. 



To ammoniacal eosin in open air 

 add picric acid to neutralization, the 

 resulting compound is an excellent 

 stain. 



245. Calberia. See No. 96. 

 Dissolve 60 pts. methyl green and 



I pt. eosin in 30% warm alcohol and 

 stain. The cuticle becomes grass- 

 green, lymph cells blue, striped mus- 

 cle red, nuclei green, unstriped mus- 

 cle green, and the intercellular sub- 

 stance red. 



The efterent ducts of the salivary 

 glands stain blue, the follicles red, 

 cells of connective tissue green or 

 greenish blue. In the sinews the 

 perichondrium becomes light green, 

 nuclei deep green. Ranvier's cells 

 medium green, and the stroma rose 

 red. 



246. See No. 99. 



The tails of young rats and mice 



are treated with silver as usual, then 

 tinged with eosin. 



247. Schiefterdecker. Kleinere his- 



tologische Mi ttheilungen 

 Arch. Mikr. Anat., xv., 30- 



40- 

 Various anil ins as dahlia, methyl- 

 violet, and green — not the methyl- 

 green of Calberia — were combined 

 with a red like eosin since 1876. The 

 smaragd green was found worthless. 

 Sections are placed first in alcohol, 

 then in a little alcoholic eosin so long 

 as required for the depth of color de- 



