1886.1 



MICROSCOPICAL JOURNAL. 



161 



sired. Wash quickly in water which 

 extracts some color, then drop in i % 

 aqueous solution of either of the 

 other dyes. When stained almost 

 black, wash in water and put in al- 

 cohol, which extracts both dyes, and 

 the exact moment should be seized 

 to remove the section when of the 

 precise shade. No directions as to 

 time can be given. Oil of cloves 

 does not extract eosin but acts slight- 

 ly on the other dyes, and should be 

 carefully removed before mounting 

 in balsam. Minute descriptions of 

 the peculiar effects of this method on 

 different organs are given. 

 24S. Tafani. Nouveau procede de 

 coloration des preparations 

 microscopiques avec une so- 

 lution picro-anilique. Journ. 

 de Microgr. 1878, p. 127-130. 

 A mixtui"e of picric acid and anilin 

 blue gives a fine stain for nuclei. 

 Add 3-4 parts of aqueous blue to 

 100 parts of picric acid in water, 

 both saturated solutions. 



249. Ehrlich. .See 102. Several ani- 



lins are combined for staining 

 the granulations of leucocytes. 



250. Barrett. .Staining fluids for 



vegetable tissues. Journ. R. 



Soc, ii, 942. 

 Plant tissues are first put in a solu- 

 tion of' Crawshaw's anilin blue,' then 

 in strong acetic acid, then in a weak 

 magenta (Judson's) , again in acetic 

 acid, and then mounted in glycerin- 

 gelatin. 



251. Gibbes. See No. 229. Gilds the 



preparations first, then stains 

 in anilin. 



252. Stirling. Reconimends 251 with 



anilin blue, iodine green, and 

 rosein. 



253. Richardson. On a blue and 



scarlet double stain, etc. Jour. 



R. Micr. Soc, i, 573-574 ^^nd 



868-872. 

 See No. 232. Sections of the 

 spinal marrow are soaked in atlas 

 scarlet, first dissolved in alcohol and 

 glycerin, then diluted with water, 

 and then in soluble anilin blue pre- 

 pared with glycerin first, and diluted 



with water. When of the proper 

 shade, lay a few minutes in water, 

 and add some glacial acetic acid, then 

 mount in balsam. No proportions 

 are given, and the process is there- 

 fore uncertain. 



254. Johne. Zur mikroskopischen 



Technik. Dtsch. Zeitschr. f. 



Thiermed. u. vergl. Path., ii. 



401-403. 

 Double staining with Gentian violet 

 and eosin or htematoxylin and picric 

 acid. The last may be mixed with 

 oil of cloves. 



255. Moore. Double staining of nu- 



cleated blood corpuscles- 

 Micr., 1 88 2, ii, 73-76, and 



256. Stowell. Coloration dift'eren- 



tielles des globules nuclees du 

 sang. The Microscope and 

 its relations to Med. and 

 Pharmacy, 1882. 

 The blood is dried on the object- 

 glass, then eosin (i to 50 water and 

 50 alcohol) and methylgreen i-ioo 

 water are poured on it. The first 

 must be allowed to dry before the 

 second is applied. Mount in balsam. 



257. Ranvier. See No.- 151. 



258. Hansen. Weiner med. Jahrb., 



1871. 

 Both the above advise to combine 

 the treatment with silver and gold. 



259. Lawdowsky, see No. 184, in- 



dependent of the two pre- 

 vious writers, appears to have 

 thought of the same combina- 

 tion. The silver treatment 

 comes first, and experiment is 

 required to determine the 

 strength of the reagents. 



260. Hoggan. Journ. de I'Anat. et 



Phys., 1879, pp. 54, 588. 

 Same as 259. 



261. Jullien. Sur une nouvelle 



methode d e coloration des 

 elements histologiques. Lyon 

 med, 1872, No. 17. 

 A mixture of indigo carmine and 

 concentrated picric acid is recom- 

 mended as a beautiful green stain, 

 coloring connective tissues blue, epi- 

 thelium yellow. Mount in glycerin. 



