1893.] MICKOSCOPICAL JOURNAL. 119 



are destroyed, but this may be avoided by making sections of 

 sputum which has been fixed and hardened. For this purpose 

 alcohol, Flemming's fluid, chromacetic acid, picric acid, and sat- 

 urated sublimate solution are well suited. Midler's fluid cannot 

 be used, as it softens and disintegrates the masses of expectorant. 



The staining solutions employed by the author were alum- 

 carmine, safranin, and hiematoxylin-eosin. By this method, in 

 three cases out of four examined, giant cells were demonstrated. 



Tubercle Bacilli Detection. — Dr. Solles suggests the follow- 

 ing method for searching for tubercle bacilli in tissue. Small 

 cubes of the tissue are colored with the two following liquids: 



I. Distilled, water, loo parts ; Prussian blue, i part ; oxalic acid, 

 3 parts. 



3. Distilled water, loo parts ; gelatin, i part. 



The two liquids are mixed and the bits of tissue immersed for 

 a sufficient time. They are then put for I3 hours in absolute 

 alcohol, 13 hours in ether, and the same time in celloidin. By this 

 method the anatomical elements are all colored, while the 

 microbes remain uncolored. He lias studied microbes in cancer 

 by the same method. 



MICROSCOPICAL NOTES. 



New List of Microscopic Sections of Rock and Minerals. 



— New and interesting specimens from Colorado, Australia, 

 New Zealand, Brazil, Hungary, Scotland, Wales, etc., i-6 each, 

 special sets for physiogiaphy. List fr^e. James R. Gregory, 88 

 • Charlotte St.. Fizrov Sq., W. London. 



Rock Sections for the Microscope. — From i-6 each : col- 

 lections of minerals for cabinets etc. Thomas D. Russell, 78 

 Newgate St.. London, E. C. 



Living Specimens for the Microscope. — Volvox globator, 

 Epistvlis flavicans, Spongilla fluviatilis ; ova of trout, showing 

 circulation of blood ; Amoeba, Vorticella, Hydra, etc., specimen 

 tube with drawing and description, i s., post free. T. E. Bolton, 

 Farley Road, Malvern Link. 



MICROSCOPICAL SOCIETIES. 



Biological and Microscopical Section, Philadelphia 

 Academy of Sciences. 



This section held, during the year 1893, nine meetings, ex- 

 clusive of those in conjimction with the Academy. The member- 

 ship has been increased by one new member, and one name 

 has been added to the list of contributors. Many interesting com- 

 munications have been made, the more important of which are : 

 On Actinomyces bovis^ by Dr. S. G. Dixon ; A Peculiarity in 



