1893.] MICROSCOPICAL JOURNAL. 203 



ehrlich's neutrophile stain. 



Distilled Water, 100 parts, 



Orauge G, (saturated watery solution,) 135 parts, 



Kubin S, (saturated watery solution,) 75 parts, 



Distilled water, 100 parts, 



Absolute Alcohol, 100 parts, 



Methyl Green, (saturated aqueous solution,) 125 parts. 



Aqua distilled, 100 parts, 



Alcohol, 100 parts, 



Glycerine, 75 parts. 



chenzinskie's kosin methyline blue solution, 

 Eosin (crystal), 1 per cent solution in 70 per cent alcohol, 20 parts. 



Methylene Blue, (saturated watery solution), 40 parts. 



Water distilled, 20 parts. 



Glycerine, 20 parts. 



The crystals, used in the preparation of these stains should be 

 strictly of selected quality as it has been found that some of 

 the same kind, but from different manufacturers, produce en- 

 tirely different effects, especially the Methylene Blue which is 

 obtainable from the one source only, where Dr. Ehrlich pro 

 cures his. 



A brief outline is here given of the manner of using these 

 stains. The steps given are as followed by Dr. Ehrlich. 



Have before you a piece of filter paper on which are placed a 

 number of carefully cleaned cover glasses. These must be very 

 thin, "extra No. 1". Wash the cover glasses first in strong 

 sulphuric acid, rinse in water and place them for a few moments 

 in glacial suli)huric acid ; they should then be washed in flow- 

 ing water until all the acid is removed, then transfered to 95 

 per cent alcohol from which they are to be taken and wiped. 



Unless the cover glasses are thin and clean no good prepara- 

 tion can be made. In case the blood is taken from man, the 

 finger is pricked with a steel pen, one of the prongs of which 

 have been broken off. From the flowing blood a very small 

 drop is caught on a cover glass near its edge and the glass 

 quickly placed, blood side downward, on another cover glass 

 which should be held in Ehrlich's Blood Cover-Glass Forcep, 

 care being taken to cover the second glass only about one half. 

 The blood will be seen to spread out between the two covers. 

 Quickly draw one cover glass from the other ; a thin layer of 

 blood will in this way be spread on both slips. Ten to twenty 



