1893.] MICROSCOPICAL JOURNAL. 255 



again, and mount in glycerine. If the exposing to the silver 

 has been short the endothelium alone is impregnated; if for a 

 longer time and to a stronger solution, the siibendothelial cells 



will be marked out. 



(To be continued.) 



Preparing Vegetable Tissue for the Microscope,- Staining. 



By ABRAHAM FLATTERS, 



MANCHESTER, ENGLAND. 



[From Report of the Manchester Microscopical Society.] 

 I._TO STAIN WITH LOGWOOD. 

 After the sections have been bleached and washed : — 1. Place 

 them in 70 per cent alcohol for 15 minutes. 2. Pour off the 

 alcohol and cover the sections with the stain, and allow them to 

 stand from 5 to 30 minutes. 3. Pour off the stain and rinse the 

 sections with 92 per cent alcohol. 4. Pour off the alcohol and 

 pass the sections through oil of cloves, and mount in balsam 

 or dammar. 



II.— TO STAIN WITH PICRO-CARMINE. 



After the sections have been bleached and washed : — 1. Place 

 them in 92 per cent alcohol for 45 minutss. 2. Pour off the al- 

 cohol, and cover them with the stain, and allow them to stand 

 from 45 to 60 minutes. 3. Pour off the stain and rinse the sections 

 with alcohol for two or three minutes. 4. Pour off the alcohol, 

 and cover the sections with an alcoholic solution of picrate of 

 ammonia, and allow them to stand for one hour. This should 

 be renewed and again allowed to stand for 45 minutes. 5. Pour 

 off the solution and rinse the sections quickly with 92 per cent 

 alcohol. 6. Pour off the alcohol, and cover the sections with oil 

 of cloves, and when clear mount in balsam. Rectified spirits and 

 absolute alcohol are sometimes recommended in staining with 

 picro-carmine, but 92 percent methylated alcohol answers very 

 well for this purpose. 



III.— TO STAIN WITH BORAX-CARMINE AND IODINE GREEN. 



After the sections have been bleached and washed : — 1. Place 

 them in 70 per cent alcohol for 15 minutes. 2. Pour off the 

 alcohol, and cover the sections with the stain, and allow them 

 to stand for 45 minutes. 3. Pour off the stain, and rins? the 

 sections with the acid-alcohol, i.e., one drop of hydrochloric-acid 

 to \ oz. alcohol. 4. Pour off the acid-alcohol, and rinse the sec- 



