1896.] MICROSCOPICAL JOURNAL. 69 



Gro^vth of Bacteria at Loav Temperature. — It is a well 

 known fact that many bacteria will retain their vitality at com- 

 paratively low temperatures. Havemann however finds that a 

 number, of micro-organisnjs are capable of growing at 7° C. 

 Complete cessation of growth at this temperature occurs in 

 Typhoid fever bacillus, Streptococcus Erysi'pelat'is, and Spirillum, 

 cholera Asiaticae. A number of organisms in the soil are capa- 

 ble of growing at 0° C (Centralblatt f Bakt. u. Parasiten Runde, 

 XVIII, 497.) 



Antitoxin Treatment. — Experiments with diptheria anti- 

 toxins in both Europe and America continue to show favorable 

 results. Dr. Paquin has aTinounced favorable results in treat- 

 ing tuberculosis with antitoxin. Mr. Roger (Centralblatt f. 

 Bakt. u. Parasiten Runde, XVIII, 637) has obtained most satis- 

 factory results in treating patients suffering with puerperal 

 fever and erysipelas by using streptococcus serum. Decided 

 improvements occurred in patients a few hours after injection. 

 Klemperer and Levy express themselves highly satisfied in the 

 treatment of typhoid fever with a serum obtained from a doo- 

 this animal showing a large amount of natural immunity. 

 The dog received large amount of virulent culture and thus in- 

 creased the potency of the serum. Experiments with guinea 

 pigs and mice indicated favorable results. In doses of 5 ccm. 

 one author's showed no indications of poisoning. Five cases of 

 typhoid fever were treated, the patients receiving 60 ccm. in- 

 jected subcutaneously. All followed a mild course and re- 

 covered. Treatment was made during the first week of the 

 disease. (Centralblatt f. Bakt. u. Parasiten Runde, XVIII p, 

 148.) 



TttEDICAL MICROSCOPY. 



The Microscopic Diagnosis of Diphtheria by a New 

 Staining-Method.— Dr. H. C. Crouch of Denver, Colo., says 

 that diphtheria bacilli, as seen in preparations from cultures 

 vary in size, the larger ones particularly presenting character- 

 istic features in the way of club-shaped ends and irregular stain- 

 ing, but all forms showing a tendency to the alteration of deeply 

 and lightly stained portions. In addition to this, and distinct 



