U6 f HEJ AMERICAN MONTHLY [April 



ventive inoculations made on the 5th and 7th of May. For 

 this experiment we inocuhated the thirty vaccinated ani- 

 mals, and also twenty-four sheep, one goat, and four cows 

 which had not received any previous treatment. 



"The very virulent virus used on the 31st of May was 

 obtained from spores preserved in my laboratory since 

 the 21st of March, 1877. 



"In order to make the experiments more comparable, 

 we inocculated alternately a vaccinated and a non-vac- 

 cinated animal. When the operation was finished, all 

 those present were invited to reasemble on June 2d — i. 

 e., forty-eight hours after the virulent inoculation was 

 made. 



"Upon the arrival of the visitors on June 2d, all were 

 astonished at the result. The twenty-four sheep, the 

 goat, and the six cows which had received the attenuated 

 virus all presented the appearance of health. On the 

 contrary, twenty of the sheep and the goat which had 

 not been vaccinated were already dead of anthrax ; two 

 more of the non-vaccinated sheep died before the eyes of 

 the spectators, and the last of the series expired before 

 the end of the day. The non vacccinnated cows were not 

 dead. We had previously proved that the cows are less 

 subject than sheep to die of anthrax. But all had an ex- 

 tensive oedema at the point of inoculation, behind the 

 shoulder. Certain of these oedematous swellings increasd 

 during the following days to such dimensions that they 

 contained several litres of liquid, deforming the animal. 

 One of them even nearly touched the earth. The tem- 

 perature of these cows was elevated 3° C. The vaccin- 

 ated cows did not experience any elevation of temper- 

 ature, or tumefaction, or the slightest loss of appetite. 

 The success, therefore, was as complete for the cows as 

 for the sheep." 



Subsequent experience has fully established the value 

 of protective inoculations in this disease, and the method 



