1896.J MICROSCOPICAL JOURNAL. 287 



fifteen to sixty minutes. 5. In Gram's iodine solution one 

 to five minutes. 6. Decolorize in anilin-oil two or three 

 hours or long-er. 7. Remove anilin-oil by blotting^-peiper, 

 mount in Canada balsam. — St Louis Med. and Surgical Jour. 



A New Method for Estimating Filicic Acid. — Dr. Kraft 

 has devised the following- method of determining- the quan- 

 tity of filicic acid present in extract of male fern: Five 

 g-m. of the extract are shaken with a solution of 2 g-m. of 

 potassium carbonate and 40 g-m. of water and 60 gm. of 

 95 per cent, alcohol for one-quarter of an hour. Eig-hty- 

 three g-m. of the mixture are filtered off immediately into 

 a separatory funnel, and to this 9 g-m. of diluted hydroch- 

 loric acid, 50 g-m. of ether and 35 g-m. of water are added 

 and the whole shaken. The aqueo-alcoholic layer is drawn 

 off, the etheral solution is ag-ain washed with 35 g-m. of 

 water, the water evaporated and the etheral solution dis- 

 tilled off in a tarred 100 ccm. Erlenmeyer flask, and finally 

 evaporated down to at least 2 g-m. by means of a hand bel- 

 lows. The residue is dissolved in 1.5 g-m. of hot anyl ac- 

 cohol, 5 g-m. of methyl alcohol added and the whole then 

 slowly precipitated by the gradual addition of 25 gm. of 

 methyl alcohol. The whole is then kept over nig-ht in a 

 closed receptacle in a cellar, filtered throug-h a tarred filter, 

 the precipitate washed with 10 ccm. of methyl alcohol at 

 60 to 70 per cent, until the residue shows no loss on heat- 

 ing-. The filicic acid thus obtained amounts to about 4 per 

 cent, of the extract. — American Drziggist. 



BACTERIOLOGY. 



Typhoid Bacilli in Pus. — Sudeck. (Munchener Med. 

 Wochenschrift, No. 21, May 26, 1896.) In an ovarian cyst 

 containing- thick pus and occuring- in a woman who had 

 had typhoid fever seven weeks previously, Sudeck was 

 able to demonstrate the typhoid bacillus both in stained 

 specimen and through culture. In the pyog-enic mem- 

 brane, however, diplococci were found and therefore the 

 author rig^htly infers that the typhoid bacilli may stand in 

 no etiologic relation to the abscess, but are there concomi- 



