1894.] MICROSCOPICAL JOURNAL. 341 



constantly, and are often filled with granules presumably 

 fat since they blacken with osmic acid. But not only fat 

 seems to be appropriated by these cells; they often con- 

 tain densely staining globules and protoplasmic masses 

 and also other smaller leucocytes. This seemed to be 

 more often the case in those found in the intestine and 

 epithelium of the mouth cavity, wheie they were often 

 quite packed with globules, some of which took a nuclear 

 others a cytoplasmic stain, and were apparently the 

 remains of leucocytes which had been engulfed. In ad- 

 dition to these large leucocytes, to which the name used 

 by Ruffer, macrophages, might be appropriately applied, 

 other leucocytes occurred, both mono and multinuclear 

 forms, and leucocytes with much or scanty protoplasm. 

 Particularly abundant, especially in the subepithelial tis- 

 sue of the mouth were small leucocytes with granules 

 which were stained a bright red, undoubtedly the eosi- 

 nophile leucocytes of Ehrlich. 



For fixing the tissue of the digestive tract mercuric 

 chloride was found to give excellent results. It was em- 

 ployed as a saturated solution in normal salt solution 

 (water 100 c. c, salt 6-10 grams). The tissue was fixed 

 in this only long enough to enable the fluid to penetrate 

 to all parts, generally one or two hours. The tissue was 

 then washed well in 67 per cent of alcohol, to which a 

 little gum camphor was added to ensure the complete 

 removal of the mercuric chloride. Tincture of iodine 

 may be used as a test for this ; which is discolored by 

 mercuric chloride. Picric alcohol also gave excellent re- 

 sults. The formula employed was as follows : 95 per 

 cent of alcohol 250 c. c, water 250 c. c, picric acid crys- 

 tals, 1 gram. Tissue was hardened in this from 12-24 

 hours, and afterwards with 67 and 82 per cent of alco- 

 hols. 



The Ehrlieh-Biondi triple satin was employed with ex- 

 cellent results. The mixture as prepared by Dr. Grubler 



